Recovery of Paracoccidioides brasiliensis from a partially calcified lymph node lesion by microaerophilic incubation of liquid media.
Sabouraudia
; 19(4): 295-300, 1981 Dec.
Article
em En
| MEDLINE
| ID: mdl-7034240
Surgical specimens were obtained from a patient who had had life-threatening paracoccidioidomycosis 5 years previously. Residual lesions were found in the mesenteric lymph nodes; there were areas of caseous necrosis separated from the surrounding tissue by a fibrous capsule made of connective tissue. Abundant P. brasiliensis cells, many of which appeared degenerated, were observed in the necrotic material. Primary isolation was possible only under the microaerophilic conditions offered by fluid media kept stationary at 36 degrees C. Cultures in solid media were obtained after serial passages and gradual accommodation of the strain to aerobic incubation. It is apparent that P. brasiliensis yeast cells can become accustomed to reduced oxygen tension in the tissues. In this way, they are able to withstand the passing of time without completely losing their viability.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Paracoccidioides
/
Paracoccidioidomicose
/
Calcinose
/
Técnicas Microbiológicas
/
Fungos
/
Linfonodos
Limite:
Adolescent
/
Adult
/
Humans
/
Male
País/Região como assunto:
America do sul
/
Brasil
Idioma:
En
Revista:
Sabouraudia
Ano de publicação:
1981
Tipo de documento:
Article
País de publicação:
Reino Unido