[Modification of the basic stain of the Ziehl-Neelsen technic in the identification of Mycobacterium leprae]. / Modificação da coloração de fundo da técnica de Ziehl-Neelsen na identificação do Mycobacterium leprae.
Hansenol Int
; 7(2): 88-94, 1982 Dec.
Article
em Pt
| MEDLINE
| ID: mdl-6198275
Authors analyse the counterstain with methylene blue solution in the usual Ziehl-Neelsen method. Considerations are made on the characteristics of the dye substance. A technique alteration is proposed: a "concomitant alkalization" of the classic aqueous methylene blue solution, by adding some drops of sodium hydroxide solution, 1:500, on the slide at the moment of the staining. By this technique it was observed: a) a larger validity period of the solution; b) an absence of precipitate in the slides; c) an easier visualization of the substrate; d) a larger contrast among the substrate and the bacilli.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Coloração e Rotulagem
/
Azul de Metileno
/
Mycobacterium leprae
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Humans
Idioma:
Pt
Revista:
Hansenol Int
Ano de publicação:
1982
Tipo de documento:
Article
País de publicação:
Brasil