A microprecipitation test for rapid detection and identification of Venezuelan, eastern and western equine encephalomyelitis viruses.
Am J Trop Med Hyg
; 24(1): 127-30, 1975 Jan.
Article
em En
| MEDLINE
| ID: mdl-46134
The development of a new diagnostic procedure for the identification of Venezvelan, eastern and western equine encephalomyelitis (VEE, EEE, WEE) viruses is described. The procedure utilizes virus precipitation with reference fluorescein-conjugated gamma globulin, followed by cellulose acetate electrophoresis. Clinical specimens containing varying concentrations of virus yielded, in primary duck embryo cell culture, sufficient virus for detection within 22 to 44 hours. Identification of VEE, EEE and WEE virus in specimens was accomplished by microprecipitation within this time. In contrast to conventional identification methods, our procedure eliminates the cost of utilizing laboratory animals and considerably reduces the time required for virus identification.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Precipitação Química
/
Vírus da Encefalite Equina Venezuelana
/
Vírus da Encefalite Equina do Oeste
/
Vírus da Encefalite
/
Encefalomielite Equina
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Animals
País/Região como assunto:
America do sul
/
Venezuela
Idioma:
En
Revista:
Am J Trop Med Hyg
Ano de publicação:
1975
Tipo de documento:
Article
País de publicação:
Estados Unidos