Separation, isolation, and immunological studies of the structural proteins of Venezuelan equine encephalomyelitis virus.
J Virol
; 14(4): 740-4, 1974 Oct.
Article
em En
| MEDLINE
| ID: mdl-4214289
Three viral proteins were separated from the TC-83 strain of Venezuelan equine encephalomyelitis virus by discontinuous polyacrylamide gel electrophoresis after disruption with sodium dodecyl sulfate and beta-2-mercaptoethanol. These proteins were inoculated into rabbits and the resultant antisera were tested for immunological activity by gel precipitation, plaque reduction neutralization, hemagglutination inhibition (HI), complement fixation, fluorescence microscopy, and mouse protection studies. All proteins were capable of stimulating precipitating antibody in rabbits, but the largest protein (VP 1), which is contained in the envelope, stimulated the production of detectable neutralizing and HI antibody against the intact virion. The other two proteins yielded little or no neutralizing or HI antibody.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Proteínas Virais
/
Vírus da Encefalite Equina Venezuelana
Limite:
Animals
País/Região como assunto:
America do sul
/
Venezuela
Idioma:
En
Revista:
J Virol
Ano de publicação:
1974
Tipo de documento:
Article
País de publicação:
Estados Unidos