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Identification of mutations in canine oral mucosal melanomas by exome sequencing and comparison with human melanomas.
Dagli, Maria Lucia Zaidan; Nagamine, Márcia Kazumi; Ikeda, Tatícia Lieh; da Fonseca, Ivone Izabel Mackowiak; Kremer, Frederico Schmitt; Seixas, Fabiana Kommling; Hernandez, Carolina Dagli; Leite, João Vitor Pereira; Yasumaru, Cassia Correa; Massoco, Cristina Oliveira; Hsieh, Ricardo; Lourenço, Silvia Vanessa; Collares, Tiago Veiras.
Afiliação
  • Dagli MLZ; Laboratory of Experimental and Comparative Oncology, Department of Pathology, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, SP, Brazil. mlzdagli@usp.br.
  • Nagamine MK; Laboratory of Experimental and Comparative Oncology, Department of Pathology, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, SP, Brazil.
  • Ikeda TL; Laboratory of Experimental and Comparative Oncology, Department of Pathology, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, SP, Brazil.
  • da Fonseca IIM; Laboratory of Experimental and Comparative Oncology, Department of Pathology, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, SP, Brazil.
  • Kremer FS; Omixlab - Laboratory of Bioinformatics, Federal University of Pelotas, Pelotas, RS, Brazil.
  • Seixas FK; Laboratory of Cancer Biotechnology, Federal University of Pelotas, Pelotas, RS, Brazil.
  • Hernandez CD; School of Pharmaceutical Sciences, University of São Paulo, São Paulo, SP, Brazil.
  • Leite JVP; Laboratory of Experimental and Comparative Oncology, Department of Pathology, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, SP, Brazil.
  • Yasumaru CC; Laboratory of Comparative Imuno-Oncology, Department of Pathology, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, SP, Brazil.
  • Massoco CO; Laboratory of Comparative Imuno-Oncology, Department of Pathology, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, SP, Brazil.
  • Hsieh R; School of Dentistry, University of São Paulo, São Paulo, SP, Brazil.
  • Lourenço SV; School of Dentistry, University of São Paulo, São Paulo, SP, Brazil.
  • Collares TV; Laboratory of Cancer Biotechnology, Federal University of Pelotas, Pelotas, RS, Brazil.
Sci Rep ; 14(1): 24174, 2024 10 15.
Article em En | MEDLINE | ID: mdl-39406779
ABSTRACT
Oral mucosal melanomas (OMMs) are aggressive neoplasms commonly found in dogs but rare in humans. Utilizing whole exome sequencing (WES), which focuses on protein-coding regions to reveal mutation profiles, we conducted a comparative analysis of canine OMM and human melanomas. This study involved DNA extraction, exome enrichment, and sequencing from three canine OMM cell lines (CMGD-2, CMGD-5, TLM-1), five canine OMM frozen samples, a human OMM cell line (MEMO), and a human commercial skin melanoma cell line (SK-MEL-28). The sequencing and subsequent analysis of FASTQ files yielded final variant files, leading to the identification of mutations. Our findings revealed a total of 500 mutated genes in canine OMM, including significant ones such as EP300, FAT4, JAK3, LRP1B, NCOR1, and NOTCH1. Notably, 82 shared mutations were identified between human melanomas and canine OMM genomes. These mutations were categorized based on the gene functions. The identification of these mutations provides critical insights that can pave the way for the development of novel therapeutic strategies for both canine and human OMM, offering hope for more effective treatments in the future.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Bucais / Sequenciamento do Exoma / Melanoma / Mucosa Bucal / Mutação Limite: Animals / Humans Idioma: En Revista: Sci Rep Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Bucais / Sequenciamento do Exoma / Melanoma / Mucosa Bucal / Mutação Limite: Animals / Humans Idioma: En Revista: Sci Rep Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido