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MicroRNA-1307-3p contributes to breast cancer progression through PRM2.
Estupiñan-Jiménez, José Roberto; Villarreal-García, Valeria; Gonzalez-Villasana, Vianey; Vivas-Mejia, Pablo E; Vazquez-Guillen, Jose Manuel; Zapata-Morin, Patricio Adrián; Flores-Colón, Marienid; Altamirano-Torres, Claudia; Viveros-Valdez, Ezequiel; Ivan, Cristina; Rashed, Mohammed H; Bayraktar, Recep; Rodríguez-Padilla, Cristina; Lopez-Berestein, Gabriel; Resendez-Perez, Diana.
Afiliação
  • Estupiñan-Jiménez JR; Departmento de Biología Celular y Genética, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, Mexico.
  • Villarreal-García V; Departmento de Biología Celular y Genética, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, Mexico.
  • Gonzalez-Villasana V; Departmento de Biología Celular y Genética, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, Mexico.
  • Vivas-Mejia PE; Department of Biochemistry, Medical Sciences Campus, University of Puerto Rico, San Juan, Puerto Rico.
  • Vazquez-Guillen JM; Comprehensive Cancer Center, Medical Sciences Campus, University of Puerto Rico, San Juan, Puerto Rico.
  • Zapata-Morin PA; Laboratorio de Inmunología y Virología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, Mexico.
  • Flores-Colón M; Laboratorio de Micología y Fitopatología, Unidad de Manipulación Genética, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, Mexico.
  • Altamirano-Torres C; Department of Biochemistry, Medical Sciences Campus, University of Puerto Rico, San Juan, Puerto Rico.
  • Viveros-Valdez E; Comprehensive Cancer Center, Medical Sciences Campus, University of Puerto Rico, San Juan, Puerto Rico.
  • Ivan C; Departmento de Biología Celular y Genética, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, Mexico.
  • Rashed MH; Departamento de Química, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, Mexico.
  • Bayraktar R; Department of Experimental Therapeutics, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA.
  • Rodríguez-Padilla C; Clinical Pharmacy Department, Faculty of Pharmacy (Boys), Al-Azhar University, Cairo, Egypt.
  • Lopez-Berestein G; Department of Translational Molecular Pathology, The University of Texas MD Anderson Cancer Center, Houston, Texas, USA.
  • Resendez-Perez D; Laboratorio de Inmunología y Virología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, Mexico.
Thorac Cancer ; 2024 Oct 09.
Article em En | MEDLINE | ID: mdl-39382427
ABSTRACT

BACKGROUND:

Despite advances in screening and therapy, breast cancer (BC) remains the predominant cancer in women globally. Dysregulation of microRNAs (miRNAs) is pivotal in carcinogenesis across various cancers, including BC. Evidence indicates that miR-1307-3p is upregulated in BC tumors, yet its target genes are not fully elucidated. This study aimed to explore how miR-1307-3p regulates BC proliferation, migration, invasion, and angiogenesis and to identify potential target genes.

METHODS:

Basal miR-1307-3p levels were quantified in BC cell lines MDA-MB-231 and MCF-7, as well as MCF-10A using quantitative real-time reverse transcription-PCR (RT-qPCR). The impact of miR-1307-3p inhibition on BC cell proliferation, migration, invasion, and angiogenesis was assessed. Nine miRNA-target prediction databases identified potential miR-1307-3p targets. Target expression was validated using RT-qPCR, Western blot, and dual-luciferase reporter assays. MiR-1307-3p was overexpressed in MDA-MB-231 and MCF-7 compared to MCF-10A.

RESULTS:

Inhibiting miR-1307-3p significantly reduced BC cell proliferation, migration, invasion, and angiogenesis. Bioinformatics analysis identified 17 potential miR-1307-3p targets, with protamine 2 (PRM2) overexpression confirmed via Western blot and dual-luciferase assays.

CONCLUSION:

MiR-1307-3p overexpression in BC promotes proliferation, migration, invasion, and angiogenesis. PRM2 emerges as a novel miR-1307-3p target in BC.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Thorac Cancer Ano de publicação: 2024 Tipo de documento: Article País de afiliação: México País de publicação: Singapura
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Thorac Cancer Ano de publicação: 2024 Tipo de documento: Article País de afiliação: México País de publicação: Singapura