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Antimicrobial and antibiofilm effect of promethazine on bacterial isolates from canine otitis externa: an in vitro study.
Guedes, Rodrigo Fonseca de Medeiros; Guedes, Gláucia Morgana de Melo; Gomes, Francisco Ivanilsom Firmiano; Soares, Ana Carla de Castro Freitas; Pereira, Vinicius Carvalho; Freitas, Alyne Soares; Amando, Bruno Rocha; Sidrim, José Júlio Costa; Cordeiro, Rossana de Aguiar; Rocha, Marcos Fábio Gadelha; Castelo-Branco, Débora de Souza Collares Maia.
Afiliação
  • Guedes RFM; Department of Pathology and Legal Medicine, Postgraduate Program in Medical Microbiology, Group of Applied Medical Microbiology, Federal University of Ceará, Rua Cel. Nunes de Melo, 1315, Rodolfo Teófilo, CEP: 60430-275, Fortaleza, Ceará, Brazil.
  • Guedes GMM; Department of Pathology and Legal Medicine, Postgraduate Program in Medical Microbiology, Group of Applied Medical Microbiology, Federal University of Ceará, Rua Cel. Nunes de Melo, 1315, Rodolfo Teófilo, CEP: 60430-275, Fortaleza, Ceará, Brazil.
  • Gomes FIF; Department of Pathology and Legal Medicine, Postgraduate Program in Medical Microbiology, Group of Applied Medical Microbiology, Federal University of Ceará, Rua Cel. Nunes de Melo, 1315, Rodolfo Teófilo, CEP: 60430-275, Fortaleza, Ceará, Brazil.
  • Soares ACCF; Department of Pathology and Legal Medicine, Postgraduate Program in Medical Microbiology, Group of Applied Medical Microbiology, Federal University of Ceará, Rua Cel. Nunes de Melo, 1315, Rodolfo Teófilo, CEP: 60430-275, Fortaleza, Ceará, Brazil.
  • Pereira VC; Department of Pathology and Legal Medicine, Postgraduate Program in Medical Microbiology, Group of Applied Medical Microbiology, Federal University of Ceará, Rua Cel. Nunes de Melo, 1315, Rodolfo Teófilo, CEP: 60430-275, Fortaleza, Ceará, Brazil.
  • Freitas AS; Department of Pathology and Legal Medicine, Postgraduate Program in Medical Microbiology, Group of Applied Medical Microbiology, Federal University of Ceará, Rua Cel. Nunes de Melo, 1315, Rodolfo Teófilo, CEP: 60430-275, Fortaleza, Ceará, Brazil.
  • Amando BR; Department of Pathology and Legal Medicine, Postgraduate Program in Medical Microbiology, Group of Applied Medical Microbiology, Federal University of Ceará, Rua Cel. Nunes de Melo, 1315, Rodolfo Teófilo, CEP: 60430-275, Fortaleza, Ceará, Brazil.
  • Sidrim JJC; Department of Pathology and Legal Medicine, Postgraduate Program in Medical Microbiology, Specialized Medical Mycology Center, Federal University of Ceará, Rua Cel. Nunes de Melo, 1315, Rodolfo Teófilo, CEP: 60430-275, Fortaleza, Ceará, Brazil.
  • Cordeiro RA; Department of Pathology and Legal Medicine, Postgraduate Program in Medical Microbiology, Specialized Medical Mycology Center, Federal University of Ceará, Rua Cel. Nunes de Melo, 1315, Rodolfo Teófilo, CEP: 60430-275, Fortaleza, Ceará, Brazil.
  • Rocha MFG; Department of Pathology and Legal Medicine, Postgraduate Program in Medical Microbiology, Specialized Medical Mycology Center, Federal University of Ceará, Rua Cel. Nunes de Melo, 1315, Rodolfo Teófilo, CEP: 60430-275, Fortaleza, Ceará, Brazil; Postgraduate Program in Veterinary Sciences, College of
  • Castelo-Branco DSCM; Department of Pathology and Legal Medicine, Postgraduate Program in Medical Microbiology, Group of Applied Medical Microbiology, Federal University of Ceará, Rua Cel. Nunes de Melo, 1315, Rodolfo Teófilo, CEP: 60430-275, Fortaleza, Ceará, Brazil; Department of Pathology and Legal Medicine, Postgradu
Microb Pathog ; 196: 106993, 2024 Nov.
Article em En | MEDLINE | ID: mdl-39374884
ABSTRACT
Otitis externa is an inflammatory disease of the external ear canal of complex and multifactorial etiology associated with recurrent bacterial infection. This study aimed to assess the antimicrobial and antibiofilm activity of promethazine against bacterial isolates from dogs with otitis externa, as well as the effect of this compound on the dynamics of biofilm formation over 120 h. Planktonic bacterial susceptibility to promethazine was evaluated to determine the minimum inhibitory concentrations (MIC). The minimum biofilm eradication concentration (MBEC) was also determined by broth microdilution. To evaluate the effect on biofilm growth, promethazine was tested at three concentrations MIC, MIC/2 and MIC/8, with daily readings at 48, 72, 96 and 120 h. The MICs of promethazine ranged from 48.83 to 781.25 µg mL-1. Promethazine significantly (P < 0.05) reduced mature biofilm biomass, with MBECs ranging from 48.8 to 6250 µg mL-1 and reduced (P < 0.01) biofilm formation for up to the 120-h, at concentrations corresponding to the MIC obtained against each isolate. Promethazine was effective against microorganisms associated with canine otitis externa. The data suggest that promethazine presents antimicrobial and antibiofilm activity and is a potential alternative to treat and prevent recurrent bacterial otitis in dogs. These results emphasize the importance of drug repurposing in veterinary otology as an alternative to reduce antimicrobial resistance.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Otite Externa / Prometazina / Testes de Sensibilidade Microbiana / Biofilmes / Doenças do Cão / Antibacterianos Limite: Animals Idioma: En Revista: Microb Pathog Assunto da revista: DOENCAS TRANSMISSIVEIS / MICROBIOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Otite Externa / Prometazina / Testes de Sensibilidade Microbiana / Biofilmes / Doenças do Cão / Antibacterianos Limite: Animals Idioma: En Revista: Microb Pathog Assunto da revista: DOENCAS TRANSMISSIVEIS / MICROBIOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido