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Molecular characterization of common zoonotic protozoan parasites and bacteria causing diarrhea in dairy calves in Ningxia Hui Autonomous Region, China.
Zhao, Jia-Qi; Fan, Ying-Ying; Lei, Yun-Duan; Liu, Ding; Wang, Jun-Wei; Yang, Xin; Song, Jun-Ke; Zhao, Guang-Hui.
Afiliação
  • Zhao JQ; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, PR China.
  • Fan YY; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, PR China.
  • Lei YD; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, PR China.
  • Liu D; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, PR China.
  • Wang JW; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, PR China.
  • Yang X; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, PR China.
  • Song JK; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, PR China.
  • Zhao GH; College of Veterinary Medicine, Northwest A&F University, Yangling 712100, PR China.
Parasite ; 31: 60, 2024.
Article em En | MEDLINE | ID: mdl-39353100
ABSTRACT
Diarrhea caused by zoonotic pathogens is one of the most common diseases in dairy calves, threatening the health of young animals. Humans are also at risk, in particular children. To explore the pathogens causing diarrhea in dairy calves, the present study applied PCR-based sequencing tools to investigate the occurrence and molecular characteristics of three parasites (Cryptosporidium spp., Giardia duodenalis, and Enterocytozoon bieneusi) and three bacterial pathogens (Escherichia coli, Clostridium perfringens, and Salmonella spp.) in 343 fecal samples of diarrheic dairy calves from five farms in Lingwu County, Ningxia Hui Autonomous Region, China. The total positive rate of these pathogens in diarrheic dairy calves was 91.0% (312/343; 95% CI, 87.9-94.0), with C. perfringens (61.5%, 211/343; 95% CI, 56.3-66.7) being the dominant one. Co-infection with two to five pathogens was found in 67.3% (231/343; 95% CI, 62.4-72.3) of investigated samples. There were significant differences (p < 0.05) in the positive rates of Cryptosporidium spp. and diarrheagenic E. coli among farms, age groups, and seasons. Two Cryptosporidium species (C. parvum and C. bovis) and five gp60 subtypes of C. parvum (IIdA15G1, IIdA20G1, IIdA19G1, IIdA14G1, and a novel IIdA13G1) were identified. Two assemblages (assemblage E and zoonotic assemblage A) of G. duodenalis and six ITS genotypes of E. bieneusi (J, Henan-IV, EbpC, I, EbpA, and ESH-01) were observed. Four virulence genes (eaeA, stx1, stx2, and st) of diarrheagenic E. coli and one toxin type (type A) of C. perfringens were detected. Our study enriches our knowledge on the characteristics and zoonotic potential of diarrhea-related pathogens in dairy calves.
Title Caractérisation moléculaire des protozoaires parasites zoonotiques courants et des bactéries responsables de diarrhée chez les veaux laitiers dans la région autonome Hui du Ningxia, en Chine. Abstract La diarrhée causée par des agents pathogènes zoonotiques est l'une des maladies les plus courantes chez les veaux laitiers, menaçant la santé des jeunes animaux. Ceci est également un risque pour la santé humaine, en particulier les enfants. Pour explorer les agents pathogènes responsables de la diarrhée chez les veaux laitiers, cette étude a utilisé des outils de séquençage basés sur la PCR pour étudier l'occurrence et les caractères moléculaires de trois parasites (Cryptosporidium spp., Giardia duodenalis et Enterocytozoon bieneusi) et de trois agents pathogènes bactériens (Escherichia coli, Clostridium perfringens et Salmonella spp.) dans 343 échantillons fécaux de veaux laitiers diarrhéiques provenant de cinq fermes du comté de Lingwu, région autonome Hui du Ningxia, en Chine. Le taux total positif de ces pathogènes chez les veaux laitiers diarrhéiques était de 91,0 % (312/343; IC à 95 %, 87,9­94,0), et C. perfringens (61,5 %, 211/343; IC à 95 %, 56,3­66,7) était le plus répandu. Une co-infection avec deux à cinq pathogènes a été trouvée dans 67,3 % (231/343; IC à 95 %, 62,4­72,3) des échantillons étudiés. Il y avait des différences significatives (p < 0,05) dans les taux positifs de Cryptosporidium spp. et d'E. coli diarrhéogènes entre les fermes, les groupes d'âge et les saisons. Deux espèces de Cryptosporidium (C. parvum et C. bovis) et cinq sous-types de gp60 de C. parvum (IIdA15G1, IIdA20G1, IIdA19G1, IIdA14G1 et un nouveau, IIdA13G1) ont été identifiés. Deux assemblages (assemblage E et assemblage zoonotique A) de G. duodenalis et six génotypes ITS d'E. bieneusi (J, Henan-IV, EbpC, I, EbpA et ESH-01) ont été observés. Quatre gènes de virulence (eaeA, stx1, stx2 et st) d'E. coli diarrhéogènes et un type de toxine (type A) de C. perfringens ont été détectés. Notre étude enrichit les connaissances sur les caractères et le potentiel zoonotique des agents pathogènes liés à la diarrhée chez les veaux laitiers.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Zoonoses / Doenças dos Bovinos / Giardia lamblia / Criptosporidiose / Cryptosporidium / Enterocytozoon / Diarreia / Fezes País/Região como assunto: Asia Idioma: En Revista: Parasite Assunto da revista: PARASITOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de publicação: França
Texto completo
Adicionar na Minha BVS
Imprimir
XML
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Zoonoses / Doenças dos Bovinos / Giardia lamblia / Criptosporidiose / Cryptosporidium / Enterocytozoon / Diarreia / Fezes País/Região como assunto: Asia Idioma: En Revista: Parasite Assunto da revista: PARASITOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de publicação: França