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SALL2 regulates neural differentiation of mouse embryonic stem cells through Tuba1a.
Xiong, Hui; Lin, Bowen; Liu, Junyang; Lu, Renhong; Lin, Zheyi; Hang, Chengwen; Liu, Wenjun; Zhang, Lei; Ding, Jie; Guo, Huixin; Zhang, Mingshuai; Wang, Siyu; Gong, Zheng; Xie, Duanyang; Liu, Yi; Shi, Dan; Liang, Dandan; Liu, Zhen; Chen, Yi-Han; Yang, Jian.
Afiliação
  • Xiong H; State Key Laboratory of Cardiovascular Diseases, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai, 200120, China.
  • Lin B; Shanghai Arrhythmia Research Center, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai, 200120, China.
  • Liu J; Department of Cardiology, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai, 200120, China.
  • Lu R; Shanghai Frontiers Center of Nanocatalytic Medicine, Shanghai, 200092, China.
  • Lin Z; Department of Cell Biology, School of Medicine, Tongji University, Shanghai, 200092, China.
  • Hang C; Clinical Center for Heart Research, Tongji University, Shanghai, 200092, China.
  • Liu W; State Key Laboratory of Cardiovascular Diseases, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai, 200120, China.
  • Zhang L; Shanghai Arrhythmia Research Center, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai, 200120, China.
  • Ding J; Department of Cardiology, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai, 200120, China.
  • Guo H; Shanghai Frontiers Center of Nanocatalytic Medicine, Shanghai, 200092, China.
  • Zhang M; Clinical Center for Heart Research, Tongji University, Shanghai, 200092, China.
  • Wang S; Department of Pathology and Pathophysiology, School of Medicine, Tongji University, Shanghai, 200092, China.
  • Gong Z; State Key Laboratory of Cardiovascular Diseases, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai, 200120, China.
  • Xie D; Shanghai Arrhythmia Research Center, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai, 200120, China.
  • Liu Y; Department of Cardiology, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai, 200120, China.
  • Shi D; Shanghai Frontiers Center of Nanocatalytic Medicine, Shanghai, 200092, China.
  • Liang D; Department of Cell Biology, School of Medicine, Tongji University, Shanghai, 200092, China.
  • Liu Z; Clinical Center for Heart Research, Tongji University, Shanghai, 200092, China.
  • Chen YH; State Key Laboratory of Cardiovascular Diseases, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai, 200120, China.
  • Yang J; Shanghai Arrhythmia Research Center, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai, 200120, China.
Cell Death Dis ; 15(9): 710, 2024 Sep 30.
Article em En | MEDLINE | ID: mdl-39349437
ABSTRACT
The spalt (Sal) gene family has four members (Sall1-4) in vertebrates, all of which play pivotal roles in various biological processes and diseases. However, the expression and function of SALL2 in development are still less clear. Here, we first charted SALL2 protein expression pattern during mouse embryo development by immunofluorescence, which revealed its dominant expression in the developing nervous system. With the establishment of Sall2 deficient mouse embryonic stem cells (ESCs), the in vitro neural differentiation system was leveraged to interrogate the function of SALL2, which showed impaired neural differentiation of Sall2 knockout (KO) ESCs. Furthermore, neural stem cells (NSCs) could not be derived from Sall2 KO ESCs and the generation of neural tube organoids (NTOs) was greatly inhibited in the absence of SALL2. Meanwhile, transgenic expression of E1 isoform of SALL2 restored the defects of neural differentiation in Sall2 KO ESCs. By chromatin immunoprecipitation sequencing (ChIP-seq), Tuba1a was identified as downstream target of SALL2, whose function in neural differentiation was confirmed by rescuing neural phenotypes of Sall2 KO ESCs when overexpressed. In sum, by elucidating SALL2 expression dynamics during early mouse development and mechanistically characterizing its indispensable role in neural differentiation, this study offers insights into SALL2's function in human nervous system development, associated pathologies stemming from its mutations and relevant therapeutic strategy.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fatores de Transcrição / Diferenciação Celular / Células-Tronco Embrionárias Murinas Limite: Animals Idioma: En Revista: Cell Death Dis Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
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XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fatores de Transcrição / Diferenciação Celular / Células-Tronco Embrionárias Murinas Limite: Animals Idioma: En Revista: Cell Death Dis Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China País de publicação: Reino Unido