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People Living With HIV Have More Intact HIV DNA in Circulating CD4+ T Cells if They Have History of Pulmonary Tuberculosis.
Juste, Marc Antoine Jean; Joseph, Yvetot; Lespinasse, Dominique; Apollon, Alexandra; Jamshidi, Parmida; Lee, Myung Hee; Ward, Maureen; Brill, Esther; Duffus, Yanique; Chukwukere, Uche; Danesh, Ali; Alberto, Winiffer Conce; Fitzgerald, Daniel W; Pape, Jean W; Jones, R Brad; Dupnik, Kathryn.
Afiliação
  • Juste MAJ; GHESKIO Centers, Port au Prince, Haiti.
  • Joseph Y; GHESKIO Centers, Port au Prince, Haiti.
  • Lespinasse D; GHESKIO Centers, Port au Prince, Haiti.
  • Apollon A; GHESKIO Centers, Port au Prince, Haiti.
  • Jamshidi P; Department of Medicine, Weill Cornell Medicine, New York, NY.
  • Lee MH; Department of Medicine, Weill Cornell Medicine, New York, NY.
  • Ward M; Department of Medicine, Weill Cornell Medicine, New York, NY.
  • Brill E; Department of Medicine, Weill Cornell Medicine, New York, NY.
  • Duffus Y; Department of Medicine, Weill Cornell Medicine, New York, NY.
  • Chukwukere U; Department of Medicine, Weill Cornell Medicine, New York, NY.
  • Danesh A; Department of Medicine, Weill Cornell Medicine, New York, NY.
  • Alberto WC; Department of Medicine, Weill Cornell Medicine, New York, NY.
  • Fitzgerald DW; Department of Medicine, Weill Cornell Medicine, New York, NY.
  • Pape JW; GHESKIO Centers, Port au Prince, Haiti.
  • Jones RB; Department of Medicine, Weill Cornell Medicine, New York, NY.
  • Dupnik K; Department of Medicine, Weill Cornell Medicine, New York, NY.
Pathog Immun ; 9(2): 172-193, 2024.
Article em En | MEDLINE | ID: mdl-39345793
ABSTRACT

Background:

A primary barrier to curing HIV is the HIV reservoir. The leading infectious cause of death worldwide for people living with HIV is tuberculosis (TB), but we do not know how TB impacts the HIV reservoir.

Methods:

Participants in identification and validation cohorts were selected from previously enrolled studies at Groupe Haïtien d'Étude du Sarcome de Kaposi et des Infections Opportunistes (GHESKIO) in Port au Prince, Haiti. Intact and non-intact proviral DNA were quantified using droplet digital PCR of peripheral blood mononuclear cell (PBMC)-derived CD4+ T cells. Kruskal-Wallis tests were used to compare medians with tobit regression for censoring.

Results:

In the identification cohort, we found that people living with HIV with a history of active pulmonary TB (n=19) had higher levels of intact provirus than people living with HIV without a history of active TB (n=47) (median 762; IQR, 183-1173 vs 117; IQR, 24-279 intact provirus per million CD4, respectively; P=0.0001). This difference also was seen in the validation cohort (n=31), (median 102; IQR, 0-737 vs 0; IQR, 0-24.5 intact provirus per million CD4, P=0.03) for TB vs no-TB history groups, respectively. The frequencies of CD4+ T cells with any detectable proviral fragment was directly proportional to the levels of interleukin-1 beta (r=0.524, P= 0.0025) and interleukin-2 (r=0.622, P=0.0002).

Conclusions:

People living with HIV with a history of active pulmonary TB have more HIV pro-virus in their circulating CD4+ T cells, even years after TB cure. We need to characterize which CD4+ T cells are harboring intact provirus to consider the impact of T cell-targeting HIV cure interventions for people living in TB-endemic areas.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Pathog Immun Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Haiti País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Pathog Immun Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Haiti País de publicação: Estados Unidos