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Development and validation of RT-LAMP for detecting yellow fever virus in non-human primates samples from Brazil.
Cardoso, Sabrina F; Yoshikawa, Andre Akira Gonzaga; Pinheiro, Iara Carolini; Granella, Lucilene Wildner; Couto-Lima, Dinair; Neves, Maycon Sebastião Alberto Santos; Mansur, Daniel Santos; Pitaluga, André N; Rona, Luísa D P.
Afiliação
  • Cardoso SF; Department of Cell Biology, Embryology, and Genetics, Federal University of Santa Catarina (UFSC), Florianópolis, Brazil.
  • Yoshikawa AAG; Directorate of Epidemiological Surveillance (DIVE), Santa Catarina's State Health Secretary, Florianópolis, Brazil.
  • Pinheiro IC; Department of Cell Biology, Embryology, and Genetics, Federal University of Santa Catarina (UFSC), Florianópolis, Brazil.
  • Granella LW; Department of Cell Biology, Embryology, and Genetics, Federal University of Santa Catarina (UFSC), Florianópolis, Brazil.
  • Couto-Lima D; Department of Microbiology, Immunology and Parasitology, Federal University of Santa Catarina (UFSC), Florianópolis, Brazil.
  • Neves MSAS; Hematozoan Transmitting Mosquito Laboratory, Oswaldo Cruz Institute (IOC), FIOCRUZ, Rio de Janeiro, RJ, Brazil.
  • Mansur DS; Hematozoan Transmitting Mosquito Laboratory, Oswaldo Cruz Institute (IOC), FIOCRUZ, Rio de Janeiro, RJ, Brazil.
  • Pitaluga AN; Department of Microbiology, Immunology and Parasitology, Federal University of Santa Catarina (UFSC), Florianópolis, Brazil.
  • Rona LDP; Oswaldo Cruz Institute (IOC), FIOCRUZ, Rio de Janeiro, RJ, Brazil. pitaluga@ioc.fiocruz.br.
Sci Rep ; 14(1): 22520, 2024 09 28.
Article em En | MEDLINE | ID: mdl-39342022
ABSTRACT
Monitoring yellow fever in non-human primates (NHPs) is an early warning system for sylvatic yellow fever outbreaks, aiding in preventing human cases. However, current diagnostic tests for this disease, primarily relying on RT-qPCR, are complex and costly. Therefore, there is a critical need for simpler and more cost-effective methods to detect yellow fever virus (YFV) infection in NHPs, enabling early identification of viral circulation. In this study, an RT-LAMP assay for detecting YFV in NHP samples was developed and validated. Two sets of RT-LAMP primers targeting the YFV NS5 and E genes were designed and tested together with a third primer set to the NS1 locus using NHP tissue samples from Southern Brazil. The results were visualized by colorimetry and compared to the RT-qPCR test. Standardization and validation of the RT-LAMP assay demonstrated 100% sensitivity and specificity compared to RT-qPCR, with a detection limit of 12 PFU/mL. Additionally, the cross-reactivity test with other flaviviruses confirmed a specificity of 100%. Our newly developed RT-LAMP diagnostic test for YFV in NHP samples will significantly contribute to yellow fever monitoring efforts, providing a simpler and more accessible method for viral early detection. This advancement holds promise for enhancing surveillance and ultimately preventing the spread of yellow fever.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Febre Amarela / Vírus da Febre Amarela / Sensibilidade e Especificidade / Técnicas de Amplificação de Ácido Nucleico Limite: Animals País/Região como assunto: America do sul / Brasil Idioma: En Revista: Sci Rep Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Febre Amarela / Vírus da Febre Amarela / Sensibilidade e Especificidade / Técnicas de Amplificação de Ácido Nucleico Limite: Animals País/Região como assunto: America do sul / Brasil Idioma: En Revista: Sci Rep Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido