Your browser doesn't support javascript.
loading
Non-Cytotoxic Graphene Nanoplatelets Upregulate Cell Proliferation and Self-Renewal Genes of Mesenchymal Stem Cells.
Nicoletti, Natália Fontana; Marinowic, Daniel Rodrigo; Perondi, Daniele; Budelon Gonçalves, João Ismael; Piazza, Diego; da Costa, Jaderson Costa; Falavigna, Asdrubal.
Afiliação
  • Nicoletti NF; Cell Therapy Laboratory (LATEC), University of Caxias do Sul (UCS), Caxias do Sul 95070-560, Brazil.
  • Marinowic DR; Cell Therapy Laboratory (LATEC), University of Caxias do Sul (UCS), Caxias do Sul 95070-560, Brazil.
  • Perondi D; Brain Institute of Rio Grande do Sul (BraIns), Pontifical Catholic University of Rio Grande do Sul (PUCRS), Porto Alegre 90610-000, Brazil.
  • Budelon Gonçalves JI; UCSGRAPHENE, University of Caxias do Sul (UCS), Caxias do Sul 95070-560, Brazil.
  • Piazza D; Brain Institute of Rio Grande do Sul (BraIns), Pontifical Catholic University of Rio Grande do Sul (PUCRS), Porto Alegre 90610-000, Brazil.
  • da Costa JC; UCSGRAPHENE, University of Caxias do Sul (UCS), Caxias do Sul 95070-560, Brazil.
  • Falavigna A; Brain Institute of Rio Grande do Sul (BraIns), Pontifical Catholic University of Rio Grande do Sul (PUCRS), Porto Alegre 90610-000, Brazil.
Int J Mol Sci ; 25(18)2024 Sep 11.
Article em En | MEDLINE | ID: mdl-39337305
ABSTRACT
Graphene nanoplatelets (UGZ-1004) are emerging as a promising biomaterial in regenerative medicine. This study comprehensively evaluates UGZ-1004, focusing on its physical properties, cytotoxicity, intracellular interactions, and, notably, its effects on mesenchymal stem cells (MSCs). UGZ-1004 was characterized by lateral dimensions and layer counts consistent with ISO standards and demonstrated a high carbon purity of 0.08%. Cytotoxicity assessments revealed that UGZ-1004 is non-toxic to various cell lines, including 3T3 fibroblasts, VERO kidney epithelial cells, BV-2 microglia, and MSCs, in accordance with ISO 10993-52020/2023 guidelines. The study focused on MSCs and revealed that UGZ-1004 supports their gene expression alterations related to self-renewal and proliferation. MSCs exposed to UGZ-1004 maintained their characteristic surface markers. Importantly, UGZ-1004 promoted significant upregulation of genes crucial for cell cycle regulation and DNA repair, such as CDK1, CDK2, and MDM2. This gene expression profile suggests that UGZ-1004 can enhance MSC self-renewal capabilities, ensuring robust cellular function and longevity. Moreover, UGZ-1004 exposure led to the downregulation of genes associated with tumor development, including CCND1 and TFDP1, mitigating potential tumorigenic risks. These findings underscore the potential of UGZ-1004 to not only bolster MSC proliferation but also enhance their self-renewal processes, which are critical for effective regenerative therapies. The study highlights the need for continued research into the long-term impacts of graphene nanoplatelets and their application in MSC-based regenerative medicine.
Assuntos
Palavras-chave
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proliferação de Células / Células-Tronco Mesenquimais / Grafite Limite: Animals Idioma: En Revista: Int J Mol Sci Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proliferação de Células / Células-Tronco Mesenquimais / Grafite Limite: Animals Idioma: En Revista: Int J Mol Sci Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça