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A Multi-Epitope Protein for High-Performance Serodiagnosis of Chronic Chagas Disease in ELISA and Lateral Flow Platforms.
Dias, Evandro R; Durans, Andressa M; Succar, Barbara B; Pinto, Luiz André L T; Lechuga, Guilherme C; Miguez, Mariana G; Figueira-Mansur, Janaina; Argondizzo, Ana P C; Bernardo, Aline R; Diniz, Rafaela L; Esteves, Gabriela S; Silva, Edimilson D; Morel, Carlos M; Borges-Pereira, José; De-Simone, Salvatore G; Junqueira, Angela C V; Provance, David William.
Afiliação
  • Dias ER; Center for Technological Development in Health, National Institute of Science and Technology for Innovation in Neglected Population Diseases, Oswald Cruz Foundation, Rio de Janeiro 21040-361, RJ, Brazil.
  • Durans AM; Tropical Medicine Graduate Program (Stricto Sensu), Oswaldo Cruz Institute, Oswald Cruz Foundation, Rio de Janeiro 21040-361, RJ, Brazil.
  • Succar BB; Laboratório de Epidemiologia e Sistemática Molecular, Oswaldo Cruz Institute, Oswald Cruz Foundation, Rio de Janeiro 21040-361, RJ, Brazil.
  • Pinto LALT; Laboratório de Doenças Parasitarias, Oswaldo Cruz Institute, Oswald Cruz Foundation, Rio de Janeiro 21040-361, RJ, Brazil.
  • Lechuga GC; Center for Technological Development in Health, National Institute of Science and Technology for Innovation in Neglected Population Diseases, Oswald Cruz Foundation, Rio de Janeiro 21040-361, RJ, Brazil.
  • Miguez MG; Laboratório de Epidemiologia e Sistemática Molecular, Oswaldo Cruz Institute, Oswald Cruz Foundation, Rio de Janeiro 21040-361, RJ, Brazil.
  • Figueira-Mansur J; Center for Technological Development in Health, National Institute of Science and Technology for Innovation in Neglected Population Diseases, Oswald Cruz Foundation, Rio de Janeiro 21040-361, RJ, Brazil.
  • Argondizzo APC; Laboratório de Epidemiologia e Sistemática Molecular, Oswaldo Cruz Institute, Oswald Cruz Foundation, Rio de Janeiro 21040-361, RJ, Brazil.
  • Bernardo AR; Center for Technological Development in Health, National Institute of Science and Technology for Innovation in Neglected Population Diseases, Oswald Cruz Foundation, Rio de Janeiro 21040-361, RJ, Brazil.
  • Diniz RL; Center for Technological Development in Health, National Institute of Science and Technology for Innovation in Neglected Population Diseases, Oswald Cruz Foundation, Rio de Janeiro 21040-361, RJ, Brazil.
  • Esteves GS; Laboratório de Epidemiologia e Sistemática Molecular, Oswaldo Cruz Institute, Oswald Cruz Foundation, Rio de Janeiro 21040-361, RJ, Brazil.
  • Silva ED; Laboratório de Tecnologia Recombinante, Institute of Technology in Immunobiology, Oswald Cruz Foundation, Rio de Janeiro 21040-361, RJ, Brazil.
  • Morel CM; Laboratório de Tecnologia Recombinante, Institute of Technology in Immunobiology, Oswald Cruz Foundation, Rio de Janeiro 21040-361, RJ, Brazil.
  • Borges-Pereira J; Laboratório de Tecnologia Recombinante, Institute of Technology in Immunobiology, Oswald Cruz Foundation, Rio de Janeiro 21040-361, RJ, Brazil.
  • De-Simone SG; Laboratório de Reativos em Diagnóstico, Institute of Technology in Immunobiology, Rio de Janeiro 21040-361, RJ, Brazil.
  • Junqueira ACV; Laboratório de Reativos em Diagnóstico, Institute of Technology in Immunobiology, Rio de Janeiro 21040-361, RJ, Brazil.
  • Provance DW; Laboratório de Tecnologia Recombinante, Institute of Technology in Immunobiology, Oswald Cruz Foundation, Rio de Janeiro 21040-361, RJ, Brazil.
Int J Mol Sci ; 25(18)2024 Sep 11.
Article em En | MEDLINE | ID: mdl-39337297
ABSTRACT
We developed a protein to rapidly and accurately diagnose Chagas disease, a life-threatening illness identified by the WHO as a critical worldwide public health risk. Limitations in present day serological tests are complicating the current health situation and contributing to most infected persons being unaware of their condition and therefore untreated. To improve diagnostic testing, we developed an immunological mimic of the etiological agent, Trypanosoma cruzi, by combining ten pathogen-specific epitopes within the beta-barrel protein structure of Thermal Green Protein. The resulting multi-epitope protein, DxCruziV3, displayed high specificity and sensitivity as the antibody capture reagent in an ELISA platform with an analytical sensitivity that exceeds WHO recommendations. Within an immunochromatographic platform, DxCruziV3 showed excellent performance for the point of application diagnosis in a region endemic for multiple diseases, the municipality of Barcelos in the state of Amazonas, Brazil. In total, 167 individuals were rapidly tested using whole blood from a finger stick. As recommended by the Brazilian Ministry of Health, venous blood samples were laboratory tested by conventional assays for comparison. Test results suggest utilizing DxCruziV3 in different assay platforms can confidently diagnose chronic infections by T. cruzi. Rapid and more accurate results will benefit everyone but will have the most noticeable impact in resource-limited rural areas where the disease is endemic.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Trypanosoma cruzi / Ensaio de Imunoadsorção Enzimática / Testes Sorológicos / Doença de Chagas / Epitopos Limite: Adult / Female / Humans / Male / Middle aged País/Região como assunto: America do sul / Brasil Idioma: En Revista: Int J Mol Sci Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Trypanosoma cruzi / Ensaio de Imunoadsorção Enzimática / Testes Sorológicos / Doença de Chagas / Epitopos Limite: Adult / Female / Humans / Male / Middle aged País/Região como assunto: America do sul / Brasil Idioma: En Revista: Int J Mol Sci Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça