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CRISPR/Cas9-Based Genome Editing of Fall Armyworm (Spodoptera frugiperda): Progress and Prospects.
Salum, Yussuf Mohamed; Yin, Anyuan; Zaheer, Uroosa; Liu, Yuanyuan; Guo, Yi; He, Weiyi.
Afiliação
  • Salum YM; State Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops, Institute of Applied Ecology, International Joint Research Laboratory of Ecological Pest Control, Ministry of Education, Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops, Fujian
  • Yin A; State Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops, Institute of Applied Ecology, International Joint Research Laboratory of Ecological Pest Control, Ministry of Education, Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops, Fujian
  • Zaheer U; State Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops, Institute of Applied Ecology, International Joint Research Laboratory of Ecological Pest Control, Ministry of Education, Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops, Fujian
  • Liu Y; State Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops, Institute of Applied Ecology, International Joint Research Laboratory of Ecological Pest Control, Ministry of Education, Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops, Fujian
  • Guo Y; Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China Ministry of Agriculture and Rural Affairs, Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Guangzhou 51
  • He W; State Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops, Institute of Applied Ecology, International Joint Research Laboratory of Ecological Pest Control, Ministry of Education, Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops, Fujian
Biomolecules ; 14(9)2024 Aug 27.
Article em En | MEDLINE | ID: mdl-39334840
ABSTRACT
The fall armyworm (Spodoptera frugiperda) poses a substantial threat to many important crops worldwide, emphasizing the need to develop and implement advanced technologies for effective pest control. CRISPR/Cas9, derived from the bacterial adaptive immune system, is a prominent tool used for genome editing in living organisms. Due to its high specificity and adaptability, the CRISPR/Cas9 system has been used in various functional gene studies through gene knockout and applied in research to engineer phenotypes that may cause economical losses. The practical application of CRISPR/Cas9 in diverse insect orders has also provided opportunities for developing strategies for genetic pest control, such as gene drive and the precision-guided sterile insect technique (pgSIT). In this review, a comprehensive overview of the recent progress in the application of the CRISPR/Cas9 system for functional gene studies in S. frugiperda is presented. We outline the fundamental principles of applying CRISPR/Cas9 in S. frugiperda through embryonic microinjection and highlight the application of CRISPR/Cas9 in the study of genes associated with diverse biological aspects, including body color, insecticide resistance, olfactory behavior, sex determination, development, and RNAi. The ability of CRISPR/Cas9 technology to induce sterility, disrupt developmental stages, and influence mating behaviors illustrates its comprehensive roles in pest management strategies. Furthermore, this review addresses the limitations of the CRISPR/Cas9 system in studying gene function in S. frugiperda and explores its future potential as a promising tool for controlling this insect pest.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Spodoptera / Sistemas CRISPR-Cas / Edição de Genes Limite: Animals Idioma: En Revista: Biomolecules Ano de publicação: 2024 Tipo de documento: Article País de publicação: Suíça
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Spodoptera / Sistemas CRISPR-Cas / Edição de Genes Limite: Animals Idioma: En Revista: Biomolecules Ano de publicação: 2024 Tipo de documento: Article País de publicação: Suíça