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The cadDX operon contributes to cadmium resistance, oxidative stress resistance, and virulence in zoonotic streptococci.
Zhu, Xinchi; Liang, Zijing; Ma, Jiale; Huang, Jinhu; Wang, Liping; Yao, Huochun; Wu, Zongfu.
Afiliação
  • Zhu X; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210014, China.
  • Liang Z; Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing, 210014, China.
  • Ma J; WOAH Reference Lab for Swine Streptococcosis, Nanjing, 210014, China.
  • Huang J; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210014, China.
  • Wang L; Key Lab of Animal Bacteriology, Ministry of Agriculture, Nanjing, 210014, China.
  • Yao H; WOAH Reference Lab for Swine Streptococcosis, Nanjing, 210014, China.
  • Wu Z; MOE Joint International Research Laboratory of Animal Health and Food Safety, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210014, China.
Vet Res ; 55(1): 119, 2024 Sep 27.
Article em En | MEDLINE | ID: mdl-39334407
ABSTRACT
Mobile genetic elements (MGEs) enable bacteria to acquire novel genes and traits. However, the functions of cargo genes within MGEs remain poorly understood. The cadmium resistance operon cadDX is present in many gram-positive bacteria. Although cadDX has been reported to be involved in metal detoxification, its regulatory mechanisms and functions in bacterial pathogenesis are poorly understood. This study revealed that cadDX contributes to cadmium resistance, oxidative stress resistance, and virulence in Streptococcus suis, an important zoonotic pathogen in pigs and humans. CadX represses cadD expression by binding to the cadDX promoter. Notably, cadX responds to H2O2 stress through an additional promoter within the cadDX operon, mitigating the harmful effect of excessive cadD expression during oxidative stress. cadDX resides within an 11 K integrative and mobilizable element that can autonomously form circular structures. Moreover, cadDX is found in diverse MGEs, accounting for its widespread distribution across various bacteria, especially among pathogenic streptococci. Transferring cadDX into another zoonotic pathogen, Streptococcus agalactiae, results in similar phenotypes, including resistance to cadmium and oxidative stresses and increased virulence of S. agalactiae in mice. The new functions and regulatory mechanisms of cadDX shed light on the importance of the cadDX system in driving evolutionary adaptations and survival strategies across diverse gram-positive bacteria.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Óperon / Infecções Estreptocócicas / Cádmio / Streptococcus suis / Estresse Oxidativo Limite: Animals Idioma: En Revista: Vet Res Assunto da revista: MEDICINA VETERINARIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Óperon / Infecções Estreptocócicas / Cádmio / Streptococcus suis / Estresse Oxidativo Limite: Animals Idioma: En Revista: Vet Res Assunto da revista: MEDICINA VETERINARIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China País de publicação: Reino Unido