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Antigenic reactivity of Leishmania (Viannia) lainsoni axenic amastigote proved to be a suitable alternative for optimizing Montenegro skin test.
de Melo, Leonardo Viana; Vasconcelos Dos Santos, Thiago; Ramos, Patrícia Karla; Lima, Luciana Vieira; Campos, Marliane Batista; Silveira, Fernando Tobias.
Afiliação
  • de Melo LV; Parasitology Department, Evandro Chagas Institute (Surveillance Secretary of Health and Environment, Ministry of Health), Ananindeua, Pará, Brazil.
  • Vasconcelos Dos Santos T; Parasitology Department, Evandro Chagas Institute (Surveillance Secretary of Health and Environment, Ministry of Health), Ananindeua, Pará, Brazil.
  • Ramos PK; Parasitology Department, Evandro Chagas Institute (Surveillance Secretary of Health and Environment, Ministry of Health), Ananindeua, Pará, Brazil.
  • Lima LV; Parasitology Department, Evandro Chagas Institute (Surveillance Secretary of Health and Environment, Ministry of Health), Ananindeua, Pará, Brazil.
  • Campos MB; Parasitology Department, Evandro Chagas Institute (Surveillance Secretary of Health and Environment, Ministry of Health), Ananindeua, Pará, Brazil.
  • Silveira FT; Parasitology Department, Evandro Chagas Institute (Surveillance Secretary of Health and Environment, Ministry of Health), Ananindeua, Pará, Brazil. fernandotobias@iec.gov.br.
Parasit Vectors ; 17(1): 402, 2024 Sep 27.
Article em En | MEDLINE | ID: mdl-39334233
ABSTRACT

BACKGROUND:

Laboratory diagnosis of American cutaneous leishmaniasis (ACL) requires a tool amenable to the epidemiological status of ACL in Brazil. Montenegro skin test (MST), an efficient immunological tool used for laboratory diagnosis of ACL, induces delayed-type hypersensitivity (DTH) response to the promastigote antigens of Leishmania; however, human immune responses against infection are modulated by the amastigote of the parasite. Leishmania (V.) lainsoni induces strong cellular immunity in humans; therefore, the antigenic reactivity of its axenic amastigote (AMA antigen) to MST was evaluated for the laboratory diagnosis of ACL.

METHODS:

Among 70 individuals examined, 60 had a laboratory-confirmed diagnosis of ACL; 53 had localized cutaneous leishmaniasis (LCL), and 7 had mucosal leishmaniasis (ML). Patients were treated at the Evandro Chagas Institute's leishmaniasis clinic, Pará State, Brazil. Ten healthy individuals with no history of ACL (control group) were also examined. Leishmania (V.) braziliensis promastigote antigen (PRO) was used to compare the reactivity with that of AMA antigen. Paired Student's t-test, kappa agreement, and Spearman test were used to evaluate the reactivity of AMA and PRO.

RESULTS:

The mean reactivity of AMA in ACL patients was 19.4 mm ± 13.3, which was higher (P < 0.001) than that of PRO 12.1 mm ± 8.1. MST reactivity according to the clinical forms revealed that AMA reactivity in LCL and ML, 18.8 mm ± 13.3 and 24.3 mm ± 13.7, was higher (P < 0.001) than that of PRO, 11.8 mm ± 8.2 and 14.6 mm ± 8.4, respectively.

CONCLUSION:

AMA reactivity was higher than that of PRO, indicating that AMA is a promising alternative for optimizing MST in the laboratory diagnosis of ACL.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Testes Cutâneos / Leishmaniose Cutânea / Leishmania / Antígenos de Protozoários Limite: Adolescent / Adult / Female / Humans / Male / Middle aged País/Região como assunto: America do sul / Brasil Idioma: En Revista: Parasit Vectors Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Testes Cutâneos / Leishmaniose Cutânea / Leishmania / Antígenos de Protozoários Limite: Adolescent / Adult / Female / Humans / Male / Middle aged País/Região como assunto: America do sul / Brasil Idioma: En Revista: Parasit Vectors Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido