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Determination of drugs of abuse and metabolites in plasma microsamples by LC-MS/MS after microQuEChERS extraction.
Smidt, Mariana; Bastiani, Marcos Frank; Hahn, Roberta Zilles; Lima Feltraco Lizot, Lilian de; Bondan, Amanda Pacheco; Peteffi, Giovana Piva; Dos Santos, Náthaly Cbf; da Costa, José Luiz; Lanaro, Rafael; Linden, Rafael.
Afiliação
  • Smidt M; Laboratory of Analytical Toxicology, Universidade Feevale, Rua Rubem Berta 200, Novo Hamburgo, CEP 93525-080, Brazil.
  • Bastiani MF; National Institute of Science and Technology in Forensic Sciences (INCT Forense), Rua Rubem Berta 200, Novo Hamburgo, CEP 93525-080, Brazil.
  • Hahn RZ; Graduate Program on Toxicology and Analytical Toxicology, Universidade Feevale, RS-239, 2755, Novo Hamburgo, CEP 93525-075, Brazil.
  • Lima Feltraco Lizot L; Laboratory of Analytical Toxicology, Universidade Feevale, Rua Rubem Berta 200, Novo Hamburgo, CEP 93525-080, Brazil.
  • Bondan AP; Laboratory of Analytical Toxicology, Universidade Feevale, Rua Rubem Berta 200, Novo Hamburgo, CEP 93525-080, Brazil.
  • Peteffi GP; Laboratory of Analytical Toxicology, Universidade Feevale, Rua Rubem Berta 200, Novo Hamburgo, CEP 93525-080, Brazil.
  • Dos Santos NC; Laboratory of Analytical Toxicology, Universidade Feevale, Rua Rubem Berta 200, Novo Hamburgo, CEP 93525-080, Brazil.
  • da Costa JL; Laboratory of Analytical Toxicology, Universidade Feevale, Rua Rubem Berta 200, Novo Hamburgo, CEP 93525-080, Brazil.
  • Lanaro R; Graduate Program on Toxicology and Analytical Toxicology, Universidade Feevale, RS-239, 2755, Novo Hamburgo, CEP 93525-075, Brazil.
  • Linden R; Centro de Informação e Assistência Toxicológica de Campinas, Universidade Estadual de Campinas (UNICAMP), Rua Vital Brasil 251, Campinas, CEP 13083-888, Brazil.
Bioanalysis ; : 1-11, 2024 Sep 27.
Article em En | MEDLINE | ID: mdl-39331130
ABSTRACT

Aim:

Identifying drugs of abuse and their metabolites in plasma is vital in both forensic and clinical toxicology. While the QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) method offers an efficient approach to sample preparation, its application is complex due to the wide-ranging properties of target analytes and the challenges posed by biological matrix interferences. This study aims to develop a microQuEChERS approach for the quantification of 14 drugs of abuse and metabolites utilizing minimal sample and solvent volumes.

Methods:

The microQuEChERS method involved using 10 µl plasma samples, 25 mg of a salt mixture and 150 µl of acetonitrile. Extracts were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS), with a 7.5 min run. The assay was validated according to bioanalytical guidelines.

Results:

The accuracy was 96.8-112.4%. The within-assay precision was within 2.0-8.9% and the between-assay precision was within 3.2-8.2%. Matrix effects were found to range from -5.7 to 13.5%. The extraction yield was higher than 74.7%.

Conclusion:

This study described a microQuEChERS sample preparation approach for determining drugs of abuse and metabolites using plasma microsamples and LC-MS/MS. The approach is efficient, environmentally friendly and suitable for scenarios with limited amounts of biological samples.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Bioanalysis Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Bioanalysis Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido