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Impact of Kluyveromyces marxianus VM004 culture conditions on the cell wall structure and its influence on aflatoxin B1 binding.
Pereyra, Carina; Del Pilar Monge, María; Bongiovanni, Silvestre; Cristofolini, Andrea; Campos, Sergio; Cavaglieri, Lilia.
Afiliação
  • Pereyra C; Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, Ruta 36, Km 601, 5800 Río Cuarto, Córdoba, Argentina; Instituto Para el Desarrollo Agroindustrial y de la Salud (IDAS), Member of Consejo Nacional de Investigaciones Científicas y Tecnológicas (CIC-CONICET), Argentina.
  • Del Pilar Monge M; Departamento de Química, Universidad Nacional de Río Cuarto, Ruta 36, Km 601, 5800 Río Cuarto, Córdoba, Argentina; Member of Consejo Nacional de Investigaciones Científicas y Tecnológicas (CIC-CONICET), Argentina.
  • Bongiovanni S; Departamento de Química, Universidad Nacional de Río Cuarto, Ruta 36, Km 601, 5800 Río Cuarto, Córdoba, Argentina; Member of Consejo Nacional de Investigaciones Científicas y Tecnológicas (CIC-CONICET), Argentina.
  • Cristofolini A; Member of Consejo Nacional de Investigaciones Científicas y Tecnológicas (CIC-CONICET), Argentina; Área de Microscopía Electrónica, Facultad de Agronomía y Veterinaria, Universidad Nacional de Río Cuarto, Ruta 36, Km 601, 5800 Río Cuarto, Córdoba, Argentina.
  • Campos S; Departamento de Microbiologia e Imunologia Veterinária, Instituto de Veterinária, Universidade Federal Rural do Rio de Janeiro, Rod. BR 465, Km 7, Seropédica, 23890-000 Rio de Janeiro, Brazil.
  • Cavaglieri L; Departamento de Microbiología e Inmunología, Universidad Nacional de Río Cuarto, Ruta 36, Km 601, 5800 Río Cuarto, Córdoba, Argentina; Member of Consejo Nacional de Investigaciones Científicas y Tecnológicas (CIC-CONICET), Argentina.
Rev Argent Microbiol ; 2024 Sep 20.
Article em En | MEDLINE | ID: mdl-39306524
ABSTRACT
The aim of this study was to determine the impact of Kluyveromyces marxianus VM004 culture conditions on the cell wall (CW) structure and its influence on aflatoxin B1 binding. The yeast was inoculated into two types of culture media yeast extract-peptone-dextrose (YPD) broth and dried distiller's grains with solubles (DDG). The CW was extracted from the biomass produced in these media. AFB1 (150ng/ml) adsorption tests using the biomass (1×107cells/ml) and the CW (0.001g) were performed at pH 2 and pH 8. Transmission electron microscopy (TEM) evaluated the CW thickness, and infrared spectroscopy (IR) determined the CW composition. Biomass production in YPD was higher than that in DDG. Cell diameter (µm) and CW thickness (µm) increased in the DDG medium. The CW percentage obtained in DDG was higher than that in YPD. The absorbance of carbohydrates by IR was higher in YPD. pH influenced AFB1 adsorption, which was lower at pH 8. The proportion of ß-glucan and chitin present in CW was higher in the YPD medium. The IR method allowed to study the CW carbohydrate variation under the influence of these carbon sources. In conclusion, the culture media composition influenced the ß-glucan and chitin composition and consequently, AFB1 adsorption.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Rev Argent Microbiol Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Argentina País de publicação: Argentina

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Rev Argent Microbiol Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Argentina País de publicação: Argentina