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Ischemic cardiac stromal fibroblast-derived protein mediators in the infarcted myocardium and transcriptomic profiling at single cell resolution.
Cha, Ed; Hong, Sung Ho; Rai, Taj; La, Vy; Madabhushi, Pranav; Teramoto, Darren; Fung, Cameron; Cheng, Pauline; Chen, Yu; Keklikian, Angelo; Liu, Jeffrey; Fang, William; Thankam, Finosh G.
Afiliação
  • Cha E; Department of Translational Research, College of Osteopathic Medicine of the Pacific, Western University of Health Sciences, 309 E. Second Street, Pomona, CA, 91766-1854, USA.
  • Hong SH; Department of Translational Research, College of Osteopathic Medicine of the Pacific, Western University of Health Sciences, 309 E. Second Street, Pomona, CA, 91766-1854, USA.
  • Rai T; Department of Translational Research, College of Osteopathic Medicine of the Pacific, Western University of Health Sciences, 309 E. Second Street, Pomona, CA, 91766-1854, USA.
  • La V; Department of Translational Research, College of Osteopathic Medicine of the Pacific, Western University of Health Sciences, 309 E. Second Street, Pomona, CA, 91766-1854, USA.
  • Madabhushi P; Department of Biology, University of California San Diego, La Jolla, CA, 92093, USA.
  • Teramoto D; Department of Translational Research, College of Osteopathic Medicine of the Pacific, Western University of Health Sciences, 309 E. Second Street, Pomona, CA, 91766-1854, USA.
  • Fung C; Department of Translational Research, College of Osteopathic Medicine of the Pacific, Western University of Health Sciences, 309 E. Second Street, Pomona, CA, 91766-1854, USA.
  • Cheng P; Department of Translational Research, College of Osteopathic Medicine of the Pacific, Western University of Health Sciences, 309 E. Second Street, Pomona, CA, 91766-1854, USA.
  • Chen Y; Molecular Instrumentation Center, University of California-Los Angeles, Los Angeles, CA, 90095, USA.
  • Keklikian A; Department of Translational Research, College of Osteopathic Medicine of the Pacific, Western University of Health Sciences, 309 E. Second Street, Pomona, CA, 91766-1854, USA.
  • Liu J; Department of Translational Research, College of Osteopathic Medicine of the Pacific, Western University of Health Sciences, 309 E. Second Street, Pomona, CA, 91766-1854, USA.
  • Fang W; Department of Translational Research, College of Osteopathic Medicine of the Pacific, Western University of Health Sciences, 309 E. Second Street, Pomona, CA, 91766-1854, USA.
  • Thankam FG; Department of Translational Research, College of Osteopathic Medicine of the Pacific, Western University of Health Sciences, 309 E. Second Street, Pomona, CA, 91766-1854, USA. FThankam@westernu.edu.
Funct Integr Genomics ; 24(5): 168, 2024 Sep 20.
Article em En | MEDLINE | ID: mdl-39302489
ABSTRACT
This article focuses on screening the major secreted proteins by the ischemia-challenged cardiac stromal fibroblasts (CF), the assessment of their expression status and functional role in the post-ischemic left ventricle (LV) and in the ischemia-challenged CF culture and to phenotype CF at single cell resolution based on the positivity of the identified mediators. The expression level of CRSP2, HSP27, IL-8, Cofilin-1, and HSP90 in the LV tissues following coronary artery bypass graft (CABG) and myocardial infarction (MI) and CF cells followed the screening profile derived from the MS/MS findings. The histology data unveiled ECM disorganization, inflammation and fibrosis reflecting the ischemic pathology. CRSP2, HSP27, and HSP90 were significantly upregulated in the LV-CABG tissues with a concomitant reduction ion LV-MI whereas Cofilin-1, IL8, Nrf2, and Troponin I were downregulated in LV-CABG and increased in LV-MI. Similar trends were exhibited by ischemic CF. Single cell transcriptomics revealed multiple sub-phenotypes of CF based on their respective upregulation of CRSP2, HSP27, IL-8, Cofilin-1, HSP90, Troponin I and Nrf2 unveiling pathological and pro-healing phenotypes. Further investigations regarding the underlying signaling mechanisms and validation of sub-populations would offer novel translational avenues for the management of cardiac diseases.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Análise de Célula Única / Fibroblastos / Infarto do Miocárdio Limite: Humans / Male Idioma: En Revista: Funct Integr Genomics Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Alemanha
Texto completo
Adicionar na Minha BVS
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Análise de Célula Única / Fibroblastos / Infarto do Miocárdio Limite: Humans / Male Idioma: En Revista: Funct Integr Genomics Assunto da revista: BIOLOGIA MOLECULAR / GENETICA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Estados Unidos País de publicação: Alemanha