Your browser doesn't support javascript.
loading
Transcriptome analysis of the effects of high temperature on zygotic genome activation in porcine embryos.
Sun, Ming-Hong; Zhan, Cheng-Lin; Li, Xiao-Han; Lee, Song-Hee; Cui, Xiang-Shun.
Afiliação
  • Sun MH; College of Animal Science and Technology, Southwest University, Chongqing, 400715, China.
  • Zhan CL; Department of Animal Science, Chungbuk National University, Cheongju, 28644, South Korea.
  • Li XH; Chongqing Key Laboratory of Herbivore Science, Chongqing, 400715, China.
  • Lee SH; Department of Animal Science, Chungbuk National University, Cheongju, 28644, South Korea.
  • Cui XS; Department of Animal Science, Chungbuk National University, Cheongju, 28644, South Korea.
Sci Rep ; 14(1): 21849, 2024 09 19.
Article em En | MEDLINE | ID: mdl-39300156
ABSTRACT
Damage to the development of porcine gametes and embryos caused by high temperatures (HT) is one of the main reasons for the decline in the economic benefits of the livestock industry. Zygotic genome activation (ZGA) marks the beginning of gene expression programs in mammalian pre-implantation embryos. In pigs, ZGA occurs at the 4-cell (4 C) stage, indicating that correct gene expression at this stage plays an important regulatory role in embryonic development. However, the effect of the HT environment on early porcine embryonic development and the RNA expression profile of ZGA remain unclear. In this study, we compared the RNA transcription patterns of porcine 4 C embryos under normal and HT conditions using RNA-seq and identified 326 differentially expressed genes (DEGs). These changes were mainly related to DNA polymerase activity, DNA replication, and nucleotidyltransferase activity. In addition, entries for reverse transcription and endonuclease activity were enriched, indicating that ZGA interfered under HT conditions. Further comparison of the experimental results with the porcine ZGA gene revealed 39 ZGA genes among the DEGs. KEGG and GSEA analysis showed that the oxidative phosphorylation pathway was significantly enriched and signaling pathways related to energy metabolism were significantly downregulated. We also found that NDUFA6 and CDKN1A were located at the center of the protein-protein interaction network diagram of the DEGs. In summary, HT conditions affect mitochondrial function and oxidative phosphorylation levels, and lead to changes in the expression pattern of ZGA in early porcine embryos, with its hub genes NDUFA6 and CDKN1A.
Assuntos
Palavras-chave
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Zigoto / Regulação da Expressão Gênica no Desenvolvimento / Perfilação da Expressão Gênica Limite: Animals Idioma: En Revista: Sci Rep Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Zigoto / Regulação da Expressão Gênica no Desenvolvimento / Perfilação da Expressão Gênica Limite: Animals Idioma: En Revista: Sci Rep Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China País de publicação: Reino Unido