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Laboratory and physiological aspects of substitute metazoan models for in vivo pharmacotoxicological analysis.
Ferreira, Paulo Michel Pinheiro; Ramos, Carla Lorena Silva; Filho, José Ivo Araújo Beserra; Conceição, Micaely Lorrana Pereira; Almeida, Mateus Lima; do Nascimento Rodrigues, Débora Caroline; Porto, Jhonatas Cley Santos; de Castro E Sousa, João Marcelo; Peron, Ana Paula.
Afiliação
  • Ferreira PMP; Laboratory of Experimental Cancerology (LabCancer), Department of Biophysics and Physiology, Federal University of Piauí, Teresina, 64049-550, Brazil. pmpf@ufpi.edu.br.
  • Ramos CLS; Laboratory of Experimental Cancerology (LabCancer), Department of Biophysics and Physiology, Federal University of Piauí, Teresina, 64049-550, Brazil.
  • Filho JIAB; Laboratory of Experimental Cancerology (LabCancer), Department of Biophysics and Physiology, Federal University of Piauí, Teresina, 64049-550, Brazil.
  • Conceição MLP; Laboratory of Experimental Cancerology (LabCancer), Department of Biophysics and Physiology, Federal University of Piauí, Teresina, 64049-550, Brazil.
  • Almeida ML; Laboratory of Experimental Cancerology (LabCancer), Department of Biophysics and Physiology, Federal University of Piauí, Teresina, 64049-550, Brazil.
  • do Nascimento Rodrigues DC; Laboratory of Experimental Cancerology (LabCancer), Department of Biophysics and Physiology, Federal University of Piauí, Teresina, 64049-550, Brazil.
  • Porto JCS; Laboratory of Experimental Cancerology (LabCancer), Department of Biophysics and Physiology, Federal University of Piauí, Teresina, 64049-550, Brazil.
  • de Castro E Sousa JM; Toxicological Genetics Research Laboratory (Lapgenic), Department of Biochemistry and Pharmacology, Federal University of Piauí, Teresina, 64049-550, Brazil.
  • Peron AP; Laboratory of Ecotoxicology (Labecotox), Department of Biodiversity and Nature Conservation, Federal Technological University of Paraná, Campo Mourão, 87301-899, Brazil.
Article em En | MEDLINE | ID: mdl-39298017
ABSTRACT
New methods are essential to characterize the performance of substitute procedures for detecting therapeutic action(s) of a chemical or key signal of toxicological events. Herein, it was discussed the applications and advantages of using arthropods, worms, and fishes in pharmacological and/or toxicology assessments. First of all, the illusion of similarity covers many differences between humans and mice, remarkably about liver injury and metabolism of xenobiotics. Using invertebrates, especially earthworms (Eisenia fetida), brine shrimps (Artemia salina, Daphnia magna), and insects (Drosophila melanogaster) and vertebrates as small fishes (Oryzias latipes, Pimephales promelas, Danio rerio) has countless advantages, including fewer ethical conflicts, short life cycle, high reproduction rate, simpler to handle, and less complex anatomy. They can be used to find contaminants in organic matters and water and are easier genetically engineered with orthologous-mutated genes to explore specific proteins involved in proliferative and hormonal disturbances, chemotherapy multidrug resistance, and carcinogenicity. As multicellular embryos, larvae, and mature organisms, they can be tested in bigger-sized replication platforms with 24-, 96-, or 384-multiwell plates as cheaper and faster ways to select hit compounds from drug-like libraries to predict acute, subacute or chronic toxicity, pharmacokinetics, and efficacy parameters of pharmaceutical, cosmetic, and personal care products. Meanwhile, sublethal exposures are designed to identify changes in reproduction, body weight, DNA damages, oxidation, and immune defense responses in earthworms and zebrafishes, and swimming behaviors in A. salina and D. rerio. Behavioral parameters also give specificities on sublethal effects that would not be detected in zebrafishes by OECD protocols.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Naunyn Schmiedebergs Arch Pharmacol Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Alemanha

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Naunyn Schmiedebergs Arch Pharmacol Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Alemanha