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Resveratrol attenuates doxorubicin-induced toxicity during in vitro culture of mouse-isolated preantral follicles.
Silva, G A L; Monte, A P O; França, A T V; Mota, I M; Oliveira Junior, J L; Andrade, K O; Souza, L M; Silva, R L S; Guimarães, V S; Barberino, R S; Palheta Junior, R C; Smitz, J E J; Matos, M H T.
Afiliação
  • Silva GAL; Nucleus of Biotechnology Applied to Ovarian Follicle Development, Department of Veterinary Medicine, Federal University of Vale do São Francisco - UNIVASF, Petrolina, Pernambuco, Brazil.
  • Monte APO; Nucleus of Biotechnology Applied to Ovarian Follicle Development, Department of Veterinary Medicine, Federal University of Vale do São Francisco - UNIVASF, Petrolina, Pernambuco, Brazil.
  • França ATV; Nucleus of Biotechnology Applied to Ovarian Follicle Development, Department of Veterinary Medicine, Federal University of Vale do São Francisco - UNIVASF, Petrolina, Pernambuco, Brazil.
  • Mota IM; Nucleus of Biotechnology Applied to Ovarian Follicle Development, Department of Veterinary Medicine, Federal University of Vale do São Francisco - UNIVASF, Petrolina, Pernambuco, Brazil.
  • Oliveira Junior JL; Nucleus of Biotechnology Applied to Ovarian Follicle Development, Department of Veterinary Medicine, Federal University of Vale do São Francisco - UNIVASF, Petrolina, Pernambuco, Brazil.
  • Andrade KO; Nucleus of Biotechnology Applied to Ovarian Follicle Development, Department of Veterinary Medicine, Federal University of Vale do São Francisco - UNIVASF, Petrolina, Pernambuco, Brazil.
  • Souza LM; Nucleus of Biotechnology Applied to Ovarian Follicle Development, Department of Veterinary Medicine, Federal University of Vale do São Francisco - UNIVASF, Petrolina, Pernambuco, Brazil.
  • Silva RLS; Nucleus of Biotechnology Applied to Ovarian Follicle Development, Department of Veterinary Medicine, Federal University of Vale do São Francisco - UNIVASF, Petrolina, Pernambuco, Brazil.
  • Guimarães VS; Nucleus of Biotechnology Applied to Ovarian Follicle Development, Department of Veterinary Medicine, Federal University of Vale do São Francisco - UNIVASF, Petrolina, Pernambuco, Brazil.
  • Barberino RS; Nucleus of Biotechnology Applied to Ovarian Follicle Development, Department of Veterinary Medicine, Federal University of Vale do São Francisco - UNIVASF, Petrolina, Pernambuco, Brazil.
  • Palheta Junior RC; Veterinary Pharmacology Laboratory, Department of Veterinary Medicine, Federal University of Vale do São Francisco - UNIVASF, Petrolina, Pernambuco, Brazil.
  • Smitz JEJ; Follicle Biology Laboratory, Free University Brussels-VUB, Brussels, Belgium.
  • Matos MHT; Nucleus of Biotechnology Applied to Ovarian Follicle Development, Department of Veterinary Medicine, Federal University of Vale do São Francisco - UNIVASF, Petrolina, Pernambuco, Brazil.
Zygote ; 32(4): 294-302, 2024 Aug.
Article em En | MEDLINE | ID: mdl-39297646
ABSTRACT
The aims of this study were to evaluate the doxorubicin concentration that induces toxic effects on in vitro culture of isolated mouse secondary follicles and to investigate whether resveratrol can inhibit or reduce this toxicity. Secondary follicles were isolated and cultured for 12 days in control medium (α-MEM+) or in α-MEM+ supplemented with doxorubicin (0.1 µg/ml) or different concentrations of resveratrol (0.5, 2, or 5 µM) associated with doxorubicin (0.1 µg/ml) (experiment 1). For experiment 2, follicles were cultured in α-MEM+ alone or supplemented with doxorubicin (0.3 µg/ml) or different concentrations of resveratrol (5 or 10 µM) associated or not with doxorubicin (0.3 µg/ml) (experiment 2). The endpoints analyzed were morphology (survival), antrum formation, follicular diameter, mitochondrial activity, glutathione (GSH) levels and DNA fragmentation. In the first experiment, doxorubicin (0.1 µg/ml) maintained survival and antrum formation similar to the control, while 5 µM resveratrol showed increased parameters, maintained mitochondrial activity and increased GSH levels compared to the control. In the second experiment, doxorubicin (0.3 µg/ml) reduced survival, antrum formation and follicular diameter compared to the control. Resveratrol at a concentration of 10 µM attenuated the damage caused by doxorubicin by improving follicular survival and did not present DNA fragmentation. In conclusion, supplementation of the in vitro culture medium with 0.3 µg/ml doxorubicin reduced the survival and impaired the development of mouse-isolated preantral follicles. Resveratrol at 10 µM reduced doxorubicin-induced follicular atresia, without DNA fragmentation in the follicles.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doxorrubicina / Resveratrol / Folículo Ovariano Limite: Animals Idioma: En Revista: Zygote Assunto da revista: EMBRIOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Doxorrubicina / Resveratrol / Folículo Ovariano Limite: Animals Idioma: En Revista: Zygote Assunto da revista: EMBRIOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido