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Lipoteichoic Acid from Heyndrickxia coagulans HOM5301 Modulates the Immune Response of RAW 264.7 Macrophages.
Zhang, Shiqi; Li, Pinglan; Zhang, Xiao; Ding, Yan; Wang, Tingting; Lee, Suwon; Xu, Ying; Lim, Chongyoon; Shang, Nan.
Afiliação
  • Zhang S; College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
  • Li P; Food & Biotech R&D Center, Coree Beijing Co., Ltd., Beijing 101312, China.
  • Zhang X; Key Laboratory of Precision Nutrition and Food Quality, Department of Nutrition and Healthy, China Agricultural University, Beijing 100083, China.
  • Ding Y; College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
  • Wang T; Food & Biotech R&D Center, Coree Beijing Co., Ltd., Beijing 101312, China.
  • Lee S; Food & Biotech R&D Center, Coree Beijing Co., Ltd., Beijing 101312, China.
  • Xu Y; Food & Biotech R&D Center, Coree Beijing Co., Ltd., Beijing 101312, China.
  • Lim C; Food & Biotech R&D Center, Coree Beijing Co., Ltd., Beijing 101312, China.
  • Shang N; Food & Biotech R&D Center, Coree Beijing Co., Ltd., Beijing 101312, China.
Nutrients ; 16(17)2024 Sep 06.
Article em En | MEDLINE | ID: mdl-39275329
ABSTRACT
Heyndrickxia coagulans (formerly Bacillus coagulans) has been increasingly utilized as an immunomodulatory probiotics. Oral administration of H. coagulans HOM5301 significantly boosted both innate and adaptive immunity in mice, particularly by increasing the phagocytic capacity of monocytes/macrophages. Lipoteichoic acid (LTA), a major microbe-associated molecular pattern (MAMP) in Gram-positive bacteria, exhibits differential immunomodulatory effects due to its structural heterogeneity. We extracted, purified, and characterized LTA from H. coagulans HOM5301. The results showed that HOM5301 LTA consists of a glycerophosphate backbone. Its molecular weight is in the range of 10-16 kDa. HOM5301 LTA induced greater productions of nitric oxide, TNFα, and IL-6 in RAW 264.7 macrophages compared to Staphylococcus aureus LTA. Comparative transcriptome and proteome analyses identified the differentially expressed genes and proteins triggered by HOM5301 LTA. KEGG analyses revealed that HOM5301 LTA transcriptionally and translationally activated macrophages through two immune-related pathways cytokine-cytokine receptor interaction and phagosome formation. Protein-protein interaction network analysis indicated that the pro-inflammatory response elicited by HOM5301 LTA was TLR2-dependent, possibly requiring the coreceptor CD14, and is mediated via the MAPK and NF-kappaB pathways. Our results demonstrate that LTA is an important MAMP of H. coagulans HOM5301 that boosts immune responses, suggesting that HOM5301 LTA may be a promising immunoadjuvant.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ácidos Teicoicos / Lipopolissacarídeos / Macrófagos Limite: Animals Idioma: En Revista: Nutrients Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China País de publicação: Suíça
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ácidos Teicoicos / Lipopolissacarídeos / Macrófagos Limite: Animals Idioma: En Revista: Nutrients Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China País de publicação: Suíça