Protocol for detecting lysosome quantity and membrane permeability in acute myeloid leukemia cell lines.
STAR Protoc
; 5(3): 103309, 2024 Sep 20.
Article
em En
| MEDLINE
| ID: mdl-39269898
ABSTRACT
Lysosomal function and activity are essential to support cellular adaptation to multiple stresses. For example, certain drugs can induce increased lysosomal membrane permeability to exert their anti-cancer effects. Here, we present a protocol to evaluate the lysosome alterations induced by drug treatment. We first describe the steps for inducing lysosomal alterations in cultured cells. We then show how to quantify the number of lysosomes, assess the integrity of lysosomal membranes, and determine lysosomal membrane permeabilization by using galectin puncta assay. For complete details on the use and execution of this protocol, please refer to Jiang et al.1.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Leucemia Mieloide Aguda
/
Permeabilidade da Membrana Celular
/
Lisossomos
Limite:
Humans
Idioma:
En
Revista:
STAR Protoc
Ano de publicação:
2024
Tipo de documento:
Article
País de publicação:
Estados Unidos