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Vitamin D treatment distinctly modulates cytokine production by peripheral blood mononuclear cells among patients with chronic cardiac and indeterminate clinical forms of Chagas disease.
Oliveira, Kamila Kássia Dos Santos; Torres, Diego José Lira; Barros, Michelle da Silva; Rafael Moreira, Leyllane; Junior, Claudeir Dias da Silva; Soares, Ana Karine de Araújo; de Albuquerque, Maria da Piedade Costa Reis; Cavalcante, Maria da Glória Aureliano Melo; Junior, Wilson Alves de Oliveira; Rabello, Michelle Christiane da Silva; de Lorena, Virginia Maria Barros.
Afiliação
  • Oliveira KKDS; Institute Aggeu Magalhães, Laboratory of Immunoparasitology, Oswaldo Cruz Foundation-FIOCRUZ, Recife, Pernambuco, Brazil.
  • Torres DJL; Institute Aggeu Magalhães, Laboratory of Immunoparasitology, Oswaldo Cruz Foundation-FIOCRUZ, Recife, Pernambuco, Brazil.
  • Barros MDS; Tropical Medicine Department, Federal University of Pernambuco (UFPE), Recife, Pernambuco, Brazil.
  • Rafael Moreira L; Institute Aggeu Magalhães, Laboratory of Immunoparasitology, Oswaldo Cruz Foundation-FIOCRUZ, Recife, Pernambuco, Brazil.
  • Junior CDDS; Institute Aggeu Magalhães, Laboratory of Immunoparasitology, Oswaldo Cruz Foundation-FIOCRUZ, Recife, Pernambuco, Brazil.
  • Soares AKA; Tropical Medicine Department, Federal University of Pernambuco (UFPE), Recife, Pernambuco, Brazil.
  • de Albuquerque MDPCR; Institute Aggeu Magalhães, Laboratory of Immunoparasitology, Oswaldo Cruz Foundation-FIOCRUZ, Recife, Pernambuco, Brazil.
  • Cavalcante MDGAM; Tropical Medicine Department, Federal University of Pernambuco (UFPE), Recife, Pernambuco, Brazil.
  • Junior WAO; Altino Ventura Foundation (FAV), Recife, Pernambuco, Brazil.
  • Rabello MCDS; Chagas disease and Heart Failure Outpatient Clinic of the Pronto Socorro Cardiológico de Pernambuco, University of Pernambuco (UPE), Recife, Pernambuco, Brazil.
  • de Lorena VMB; Chagas disease and Heart Failure Outpatient Clinic of the Pronto Socorro Cardiológico de Pernambuco, University of Pernambuco (UPE), Recife, Pernambuco, Brazil.
Immun Inflamm Dis ; 12(9): e1330, 2024 Sep.
Article em En | MEDLINE | ID: mdl-39267468
ABSTRACT

INTRODUCTION:

Chagas disease is caused by the protozoan Trypanosoma cruzi and is clinically divided into acute and chronic phases. Chronic Chagas cardiomyopathy is the most studied manifestation of the disease. Vitamin D deficiency has been suggested as a risk factor for cardiovascular disease. No studies demonstrate the action of this hormone in the cells of patients with chronic Chagas heart disease.

OBJECTIVE:

To evaluate the in vitro immunomodulatory effect of vitamin D on peripheral blood mononuclear cells of patients with the different chronic clinical forms of Chagas disease. Evaluating vitamin D's in vitro effect on blood cells by producing cytokines.

METHODS:

Thirteen patients of the undetermined form (IND), 13 of the mild cardiac form (CARD1) and 14 of the severe cardiac form (CARD2) of Chagas disease, and 12 with idiopathic heart disease (CARDid) were included. The cells obtained from peripheral blood were treated in vitro with vitamin D (1 × 10-7 M) for 24 h and cytokines were dosed in the culture supernatant.

RESULTS:

Although it was not possible to demonstrate statistically significant differences between the groups studied, our data showed that the cells treated with vitamin D modify (p < .05) the production of interferon-γ (IFN-γ) (decrease in IND), tumor necrosis factor-α (TNF-α) (decreased in CARD1 and CARDid), interleukin (IL)-6 (increased in all groups), and IL-10 (decreased in CARD1, CARD2, and CARDid) when compared to untreated cells.

CONCLUSION:

In vitro treatment with vitamin D distinctly modulated the production of cytokines by mononuclear cells of peripheral blood among patients with chronic and indeterminate cardiac clinical forms of Chagas disease.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vitamina D / Leucócitos Mononucleares / Citocinas Limite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Immun Inflamm Dis Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vitamina D / Leucócitos Mononucleares / Citocinas Limite: Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Immun Inflamm Dis Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido