Development of an alternative method to quantify H2S: application in wine fermentation.
J Sci Food Agric
; 2024 Sep 12.
Article
em En
| MEDLINE
| ID: mdl-39264031
ABSTRACT
BACKGROUND:
A colorimetric method for the quantification of hydrogen sulfide (H2S) produced in microbial fermentations was developed using lead gelled alginate microparticles packed in glass columns. The formation of a lead sulfide complex, between H2S and lead ion (Pb2+) immobilized on the microparticles, allowed simple and accurate quantification by colorimetry.RESULTS:
The microparticle-loaded columns were calibrated and showed significant analytical sensitivity. The calibration curve of the system showed a correlation coefficient (r2) of 0.995 and a detection limit of 1.29 ± 0.02 µg L-1. The application of the columns in laboratory wine fermentations was able to detect variations in H2S production from 10.6 to 23.5 µg L-1 by increasing the sugar content in the medium, and from 10.6 to 3.2 µg L-1 with decreasing nitrogen content in the medium.CONCLUSION:
Validation of the proposed method was carried out by determining H2S in a vinic fermentation model, the results of which were compared with those obtained using a reference chemical method. The data obtained showed no statistically significant differences between the two methods, confirming the reliability and accuracy of the developed system. © 2024 Society of Chemical Industry.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
J Sci Food Agric
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
Chile
País de publicação:
Reino Unido