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Modulating pro-fibrotic macrophages using yeast beta-glucan microparticles prepared by Pressurized Gas eXpanded liquid (PGX) Technology®.
Naiel, S; Dowdall, N; Zhou, Q; Ali, P; Hayat, A; Vierhout, M; Wong, E Y; Couto, R; Yépez, B; Seifried, B; Moquin, P; Kolb, M R; Ask, K; Hoare, T.
Afiliação
  • Naiel S; Department of Medicine, Firestone Institute for Respiratory Health, McMaster University, 50 Charlton Avenue East, L314-5, Hamilton, ON, L8N 4A6, Canada.
  • Dowdall N; Department of Chemical Engineering, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L8, Canada.
  • Zhou Q; Department of Pathology and Molecular Medicine, McMaster Immunology Research Center, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L8, Canada.
  • Ali P; Department of Medicine, Firestone Institute for Respiratory Health, McMaster University, 50 Charlton Avenue East, L314-5, Hamilton, ON, L8N 4A6, Canada.
  • Hayat A; Department of Medicine, Firestone Institute for Respiratory Health, McMaster University, 50 Charlton Avenue East, L314-5, Hamilton, ON, L8N 4A6, Canada.
  • Vierhout M; Department of Medicine, Firestone Institute for Respiratory Health, McMaster University, 50 Charlton Avenue East, L314-5, Hamilton, ON, L8N 4A6, Canada.
  • Wong EY; Ceapro Inc., 7824 51 Ave NW, Edmonton, AB, T6E 6W2, Canada.
  • Couto R; Ceapro Inc., 7824 51 Ave NW, Edmonton, AB, T6E 6W2, Canada.
  • Yépez B; Ceapro Inc., 7824 51 Ave NW, Edmonton, AB, T6E 6W2, Canada.
  • Seifried B; Ceapro Inc., 7824 51 Ave NW, Edmonton, AB, T6E 6W2, Canada.
  • Moquin P; Ceapro Inc., 7824 51 Ave NW, Edmonton, AB, T6E 6W2, Canada.
  • Kolb MR; Department of Pathology and Molecular Medicine, McMaster Immunology Research Center, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L8, Canada.
  • Ask K; Department of Medicine, Firestone Institute for Respiratory Health, McMaster University, 50 Charlton Avenue East, L314-5, Hamilton, ON, L8N 4A6, Canada. Electronic address: askkj@mcmaster.ca.
  • Hoare T; Department of Chemical Engineering, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L8, Canada. Electronic address: hoaretr@mcmaster.ca.
Biomaterials ; 313: 122816, 2025 Feb.
Article em En | MEDLINE | ID: mdl-39250864
ABSTRACT
Pro-fibrotic M2-like macrophages are widely implicated in the pathogenesis and progression of lung fibrosis due to their production of pro-fibrotic growth factors and cytokines. Yeast beta-glucan (YBG) microparticles have shown potential as immunomodulators that can convert macrophage polarization from a pro-fibrotic phenotype to an anti-fibrotic phenotype through the engagement of the Dectin-1 receptor. However, the processing conditions used to fabricate YBG microparticles can lead to unpredictable immunomodulatory effects. Herein, we report the use of Pressurized Gas eXpanded liquids (PGX) Technology® to fabricate YBG (PGX-YBG) microparticles with higher surface areas, lower densities, and smaller and more uniform size distributions compared to commercially available spray-dried YBGs. PGX-YBG is shown to activate Dectin-1 more efficiently in vitro while avoiding significant TLR 2/4 activation. Furthermore, PGX-YBG microparticles effectively modulate M2-like fibrosis-inducing murine and human macrophages into fibrosis-suppressing macrophages both in vitro as well as in ex vivo precision-cut murine lung slices, suggesting their potential utility as a therapeutic for addressing a broad spectrum of fibrotic end-point lung diseases.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Beta-Glucanas / Macrófagos Limite: Animals / Humans Idioma: En Revista: Biomaterials Ano de publicação: 2025 Tipo de documento: Article País de afiliação: Canadá País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Beta-Glucanas / Macrófagos Limite: Animals / Humans Idioma: En Revista: Biomaterials Ano de publicação: 2025 Tipo de documento: Article País de afiliação: Canadá País de publicação: Holanda