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Quality assurance of serologic testing for Chagas disease in a primary care setting of rural Paraguay.
Gabaldón-Figueira, Juan Carlos; Losada-Galvan, Irene; Rolón, Miriam; Ardiles-Ruesjas, Sofía; Chena, Lilian; Cubilla, Zully; Lesmo, Vidalia; Martínez-Peinado, Nieves; Vega, Celeste; de Arias, Antonieta Rojas; Schill, Claudia Huber; Gascón, Joaquim; Pinazo, María-Jesús; Alonso-Padilla, Julio.
Afiliação
  • Gabaldón-Figueira JC; Barcelona Institute for Global Health (ISGlobal), Barcelona, Spain; Facultat de Medicina i Ciències de la Salut, Universitat de Barcelona (UB), Barcelona, Spain.
  • Losada-Galvan I; Barcelona Institute for Global Health (ISGlobal), Barcelona, Spain; Facultat de Medicina i Ciències de la Salut, Universitat de Barcelona (UB), Barcelona, Spain.
  • Rolón M; Centro Para El Desarrollo de la Investigación Científica (CEDIC), Manduvirá, 635 CP1255, Asunción, Paraguay.
  • Ardiles-Ruesjas S; Barcelona Institute for Global Health (ISGlobal), Barcelona, Spain; Institute of Medical Microbiology and Hygiene, Saarland University, Homburg, Saarland, Germany.
  • Chena L; Departamento de Parasitología, Ministerio de Salud Pública y Bienestar Social, Laboratorio Central de Salud Pública, Paraguay.
  • Cubilla Z; Ministerio de Salud Pública y Bienestar Social, Centro de Salud Teniente Irala, Fernández, Paraguay.
  • Lesmo V; Programa Nacional de Control de la Enfermedad de Chagas (SENEPA), Asunción, Paraguay.
  • Martínez-Peinado N; Barcelona Institute for Global Health (ISGlobal), Barcelona, Spain; Secció de Parasitologia, Departament de Biologia, Sanitat i Medi Ambient, Facultat de Farmàcia i Ciències de l'Alimentació, Universitat de Barcelona, Barcelona 08007, Spain.
  • Vega C; Centro Para El Desarrollo de la Investigación Científica (CEDIC), Manduvirá, 635 CP1255, Asunción, Paraguay.
  • de Arias AR; Centro Para El Desarrollo de la Investigación Científica (CEDIC), Manduvirá, 635 CP1255, Asunción, Paraguay.
  • Schill CH; Departamento de Parasitología, Ministerio de Salud Pública y Bienestar Social, Laboratorio Central de Salud Pública, Paraguay.
  • Gascón J; Barcelona Institute for Global Health (ISGlobal), Barcelona, Spain; CIBER de Enfermedades Infecciosas, Instituto de Salud Carlos III (CIBERINFEC, ISCIII), Madrid, Spain.
  • Pinazo MJ; CIBER de Enfermedades Infecciosas, Instituto de Salud Carlos III (CIBERINFEC, ISCIII), Madrid, Spain; Drugs for Neglected Diseases Initiative (DNDi), Rio de Janeiro, Brazil.
  • Alonso-Padilla J; Barcelona Institute for Global Health (ISGlobal), Barcelona, Spain; CIBER de Enfermedades Infecciosas, Instituto de Salud Carlos III (CIBERINFEC, ISCIII), Madrid, Spain. Electronic address: julio.a.padilla@isglobal.org.
Acta Trop ; 259: 107382, 2024 Nov.
Article em En | MEDLINE | ID: mdl-39244140
ABSTRACT
The diagnosis of Chagas disease mostly relies on the use of multiple serologic tests that are often unavailable in many of the remote settings where the disease is highly prevalent. In the Teniente Irala Fernández Municipality, in central Paraguay, efforts have been made to increase the diagnostic capabilities of specific rural health centres, but no quality assurance of the results produced has been performed. We comparatively analysed the results obtained with 300 samples tested using a commercial rapid diagnostic test (RDT) and enzyme linked immunosorbent assays (ELISA) at the laboratory of the Teniente Irala Fernández Health Center (CSTIF) with those generated upon repeating the tests at an independent well-equipped research laboratory (CEDIC). A subgroup of 52 samples were further tested at Paraguay's Central Public Health Laboratory (LCSP) by means of a different technique to evaluate the diagnostic performance of the tests carried out at CSTIF. We observed an excellent agreement between the ELISA results obtained at CSTIF and CEDIC (kappa coefficients between 0.85 and 0.93 for every kit evaluated), and an overall good performance of the tests carried out at CSTIF. However, the sensitivity of one kit was lower at CSTIF (81.3 %) than at CEDIC (100 %). The individual use of an RDT to detect the infection at CSTIF showed a similar sensitivity to that obtained combining it to an ELISA test (92.3% vs 88.5, p = 1). Nonetheless, the generalizability of this result is yet limited and will require of further studies.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Atenção Primária à Saúde / População Rural / Testes Sorológicos / Sensibilidade e Especificidade / Doença de Chagas Limite: Adolescent / Adult / Aged / Child / Child, preschool / Female / Humans / Male / Middle aged País/Região como assunto: America do sul / Paraguay Idioma: En Revista: Acta Trop Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Espanha País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Atenção Primária à Saúde / População Rural / Testes Sorológicos / Sensibilidade e Especificidade / Doença de Chagas Limite: Adolescent / Adult / Aged / Child / Child, preschool / Female / Humans / Male / Middle aged País/Região como assunto: America do sul / Paraguay Idioma: En Revista: Acta Trop Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Espanha País de publicação: Holanda