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High-throughput fluorescence lifetime imaging flow cytometry.
Kanno, Hiroshi; Hiramatsu, Kotaro; Mikami, Hideharu; Nakayashiki, Atsushi; Yamashita, Shota; Nagai, Arata; Okabe, Kohki; Li, Fan; Yin, Fei; Tominaga, Keita; Bicer, Omer Faruk; Noma, Ryohei; Kiani, Bahareh; Efa, Olga; Büscher, Martin; Wazawa, Tetsuichi; Sonoshita, Masahiro; Shintaku, Hirofumi; Nagai, Takeharu; Braun, Sigurd; Houston, Jessica P; Rashad, Sherif; Niizuma, Kuniyasu; Goda, Keisuke.
Afiliação
  • Kanno H; Department of Chemistry, The University of Tokyo, Tokyo, Japan. hkanno@g.ecc.u-tokyo.ac.jp.
  • Hiramatsu K; Department of Neurosurgical Engineering and Translational Neuroscience, Tohoku University Graduate School of Medicine, Miyagi, Japan. hkanno@g.ecc.u-tokyo.ac.jp.
  • Mikami H; Department of Chemistry, The University of Tokyo, Tokyo, Japan.
  • Nakayashiki A; Department of Chemistry, Kyushu University, Fukuoka, Japan.
  • Yamashita S; Department of Chemistry, The University of Tokyo, Tokyo, Japan.
  • Nagai A; Research Institute for Electronic Science, Hokkaido University, Hokkaido, Japan.
  • Okabe K; Department of Neurosurgery, Tohoku University Graduate School of Medicine, Miyagi, Japan.
  • Li F; Department of Neurosurgery, Tohoku University Graduate School of Medicine, Miyagi, Japan.
  • Yin F; Department of Neurosurgery, Tohoku University Graduate School of Medicine, Miyagi, Japan.
  • Tominaga K; Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, Japan.
  • Bicer OF; Department of Chemistry, The University of Tokyo, Tokyo, Japan.
  • Noma R; Department of Neurosurgical Engineering and Translational Neuroscience, Tohoku University Graduate School of Medicine, Miyagi, Japan.
  • Kiani B; Department of Neurosurgery, Tohoku University Graduate School of Medicine, Miyagi, Japan.
  • Efa O; Department of Chemistry, The University of Tokyo, Tokyo, Japan.
  • Büscher M; SANKEN (The Institute of Scientific and Industrial Research), Osaka University, Osaka, Japan.
  • Wazawa T; Miltenyi Biotec B.V. & Co. KG, Bergisch Gladbach, Germany.
  • Sonoshita M; Miltenyi Biotec B.V. & Co. KG, Bergisch Gladbach, Germany.
  • Shintaku H; Miltenyi Biotec B.V. & Co. KG, Bergisch Gladbach, Germany.
  • Nagai T; SANKEN (The Institute of Scientific and Industrial Research), Osaka University, Osaka, Japan.
  • Braun S; Institute for Genetic Medicine, Hokkaido University, Hokkaido, Japan.
  • Houston JP; Institute for Life and Medical Sciences, Kyoto University, Kyoto, Japan.
  • Rashad S; SANKEN (The Institute of Scientific and Industrial Research), Osaka University, Osaka, Japan.
  • Niizuma K; Institute for Genetics, Justus-Liebig-University Giessen, Giessen, Germany.
  • Goda K; Department of Chemical and Materials Engineering, New Mexico State University, Las Cruces, NM, USA.
Nat Commun ; 15(1): 7376, 2024 Sep 04.
Article em En | MEDLINE | ID: mdl-39231964
ABSTRACT
Flow cytometry is a vital tool in biomedical research and laboratory medicine. However, its accuracy is often compromised by undesired fluctuations in fluorescence intensity. While fluorescence lifetime imaging microscopy (FLIM) bypasses this challenge as fluorescence lifetime remains unaffected by such fluctuations, the full integration of FLIM into flow cytometry has yet to be demonstrated due to speed limitations. Here we overcome the speed limitations in FLIM, thereby enabling high-throughput FLIM flow cytometry at a high rate of over 10,000 cells per second. This is made possible by using dual intensity-modulated continuous-wave beam arrays with complementary modulation frequency pairs for fluorophore excitation and acquiring fluorescence lifetime images of rapidly flowing cells. Moreover, our FLIM system distinguishes subpopulations in male rat glioma and captures dynamic changes in the cell nucleus induced by an anti-cancer drug. FLIM flow cytometry significantly enhances cellular analysis capabilities, providing detailed insights into cellular functions, interactions, and environments.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Citometria de Fluxo / Glioma Limite: Animals / Humans / Male Idioma: En Revista: Nat Commun Assunto da revista: BIOLOGIA / CIENCIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Japão País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Citometria de Fluxo / Glioma Limite: Animals / Humans / Male Idioma: En Revista: Nat Commun Assunto da revista: BIOLOGIA / CIENCIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Japão País de publicação: Reino Unido