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Antimicrobial susceptibility of Peruvian Yersinia ruckeri isolates to oxytetracycline and florfenicol using MIC testing.
Medina-Morillo, Marco; Irgang, Rute; Yunis-Aguinaga, Jefferson; Avendaño-Herrera, Ruben.
Afiliação
  • Medina-Morillo M; Laboratorio de Patobiología Acuática, Instituto del Mar del Perú (IMARPE), Callao, Peru.
  • Irgang R; Universidad Andrés Bello, Laboratorio de Patología de Organismos Acuáticos y Biotecnología Acuícola, Facultad de Ciencias de la Vida, Viña del Mar, Chile.
  • Yunis-Aguinaga J; Interdisciplinary Center for Aquaculture Research (INCAR), Viña del Mar, Chile.
  • Avendaño-Herrera R; Laboratorio de Patobiología Acuática, Instituto del Mar del Perú (IMARPE), Callao, Peru.
Dis Aquat Organ ; 159: 127-131, 2024 Aug 29.
Article em En | MEDLINE | ID: mdl-39206607
ABSTRACT
Yersiniosis, caused by Yersinia ruckeri, has become the most common disease in farmed rainbow trout Oncorhynchus mykiss in Peru, affecting Puno and Junín Regions, important aquaculture areas in the country. Florfenicol (FLO) and oxytetracycline (OXY) are the antimicrobials most frequently used to mitigate losses attributed to this pathogen. This study presents an analysis of the susceptibility patterns of 60 Y. ruckeri isolates (30 isolates each from Puno and Junín), including the type strain CECT 4319T and the strains CECT 955 and CECT 956, against FLO and OXY. Minimum inhibitory concentrations (MICs) were determined following the guideline for standard broth dilution method published by the Clinical and Laboratory Standards Institute. MIC results ranged from 4.0 to 8.0 µg ml-1 for FLO and 0.5 to 4.0 µg ml-1 for OXY. Normalized resistance interpretation (NRI) analysis identified epidemiological cut-off values of ≤16.0 µg ml-1 for FLO and ≤4.0 µg ml-1 for OXY. All Peruvian isolates, including the collection strains, were categorized as wild-type for both antimicrobials. Even though the number of Y. ruckeri isolates with MIC values of 8 µg ml-1 for FLO is more than double in Puno than in Junín (15 vs. 7 isolates), the NRI analysis showed the same epidemiological cutoff of 16 µg ml-1; while for OXY, it was 4.0 µg ml-1 for Puno and 2.0 µg ml-1 for Junín. This study establishes the basis for monitoring susceptibility to FLO and OXY in new Y. ruckeri isolates in Peruvian rainbow trout farming.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oxitetraciclina / Tianfenicol / Testes de Sensibilidade Microbiana / Yersinia ruckeri / Doenças dos Peixes / Antibacterianos Limite: Animals País/Região como assunto: America do sul / Peru Idioma: En Revista: Dis Aquat Organ Assunto da revista: BIOLOGIA / MEDICINA VETERINARIA / MICROBIOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Peru País de publicação: Alemanha
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oxitetraciclina / Tianfenicol / Testes de Sensibilidade Microbiana / Yersinia ruckeri / Doenças dos Peixes / Antibacterianos Limite: Animals País/Região como assunto: America do sul / Peru Idioma: En Revista: Dis Aquat Organ Assunto da revista: BIOLOGIA / MEDICINA VETERINARIA / MICROBIOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Peru País de publicação: Alemanha