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Antimicrobial Effect of Chitosan Nanoparticles and Allium Species on Mycobacterium tuberculosis and Several Other Microorganisms.
Olivas-Flores, Jocelyn; Chávez-Méndez, José Román; Castillo-Martínez, Nydia Alejandra; Sánchez-Pérez, Héctor Javier; Serrano-Medina, Aracely; Cornejo-Bravo, José Manuel.
Afiliação
  • Olivas-Flores J; Faculty of Chemical Sciences and Engineering, Autonomous University of Baja California, Calzada Universidad 14418, Parque Industrial Internacional, Tijuana 22424, Mexico.
  • Chávez-Méndez JR; Faculty of Health Sciences, Autonomous University of Baja California, Blvd Universitario No. 1000, Valle San Pedro, Tijuana 21500, Mexico.
  • Castillo-Martínez NA; Faculty of Health Sciences, Autonomous University of Baja California, Blvd Universitario No. 1000, Valle San Pedro, Tijuana 21500, Mexico.
  • Sánchez-Pérez HJ; Department of Health, El Colegio de la Frontera Sur (ECOSUR), Mexican Network for Research in Tuberculosis and Other Mycobacterioses, San Cristóbal de Las Casas 29290, Mexico.
  • Serrano-Medina A; Faculty of Medicine and Psychology, Autonomous University of Baja California, Calzada Universidad 14418, Parque Industrial Internacional, Tijuana 22424, Mexico.
  • Cornejo-Bravo JM; Faculty of Chemical Sciences and Engineering, Autonomous University of Baja California, Calzada Universidad 14418, Parque Industrial Internacional, Tijuana 22424, Mexico.
Microorganisms ; 12(8)2024 Aug 06.
Article em En | MEDLINE | ID: mdl-39203447
ABSTRACT
This study evaluates the antimicrobial efficacy of chitosan nanoparticles (CNPs), varying in size, against clinical isolates of Mycobacterium tuberculosis (MTB), E. coli, S. aureus, E. faecalis, and C. albicans, as well as the antimicrobial effects of aqueous extracts and lyophilized powders of Allium (garlic) species. CNPs were synthesized through ionotropic gelation and characterized by Z potential, hydrodynamic diameter (dynamic light scattering, DLS), and SEM. Aqueous garlic extracts were prepared via decoction. We assessed antimicrobial activity using disk diffusion and broth microdilution methods; in addition, a modified agar proportion method in blood agar was used for antimicrobial activity against MTB. CNPs inhibited MTB growth at 300 µg for 116.6 nm particles and 400 µg for 364.4 nm particles. The highest antimicrobial activity was observed against E. faecalis with nanoparticles between 200 and 280 nm. Allium sativum extract produced inhibition for C. albicans at 100 µg. The results indicate that CNPs possess significant antimicrobial properties against a range of pathogens, including MTB, at high concentrations. On the other hand, aqueous Allium sativum extracts exhibited antimicrobial activity. Nonetheless, due to their instability in solution, the use of lyophilized Allium sativum powder is preferable.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Microorganisms Ano de publicação: 2024 Tipo de documento: Article País de afiliação: México País de publicação: Suíça

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Microorganisms Ano de publicação: 2024 Tipo de documento: Article País de afiliação: México País de publicação: Suíça