Reconsidering Neurogenetic Indication in the Human Brain: Broad Expression of Doublecortin Transcript in the Hippocampal and Cortical Cell Populations.
Cells Tissues Organs
; 213(5): 382-389, 2024.
Article
em En
| MEDLINE
| ID: mdl-39191219
ABSTRACT
INTRODUCTION:
Neurogenesis in the adult brain may play an important role in memory and cognition; however, knowledge of neurogenic markers in the human brain remains limited. We compared the single-nucleus transcriptome of the hippocampus with that of other cortical regions to identify hippocampus-specific neurogenic markers.METHODS:
We analyzed 26,189 nuclei from four human brains collected within 16 h of death. Clustering and annotation were performed to examine differential expression, gene ontology, and intercellular communication. DCX expression was validated by ddPCR.RESULTS:
Immature markers such as DCX, CALB2, NES, SOX2, PAX6, DPYSL3, and TUBB3 were expressed in both hippocampus and prefrontal cortex, with higher levels in the prefrontal cortex. ddPCR confirmed higher expression of DCX in the prefrontal cortex. DCX was involved in both neurogenesis and neuroprotection pathways.CONCLUSION:
Neurogenic markers are not definitive indicators of adult neurogenesis as their roles are more complex than previously understood.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neurogênese
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Proteína Duplacortina
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Hipocampo
Limite:
Adult
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Female
/
Humans
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Male
Idioma:
En
Revista:
Cells Tissues Organs
Assunto da revista:
ANATOMIA
Ano de publicação:
2024
Tipo de documento:
Article
País de publicação:
Suíça