Your browser doesn't support javascript.
loading
Microbicidal Polymer Nanoparticles Containing Clotrimazole for Treatment of Vulvovaginal Candidiasis.
Del Rocío Lara-Sánchez, María; Ganem-Rondero, Adriana; Nava-Arzaluz, María Guadalupe; Becerril-Osnaya, Andrea Angela; Pérez-Carranza, Laura Abril; Alcalá-Alcalá, Sergio; Mendoza-Muñoz, Néstor; Piñón-Segundo, Elizabeth.
Afiliação
  • Del Rocío Lara-Sánchez M; Laboratorio de Sistemas Farmacéuticos de Liberación Modificada (L-13, UIM), Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, 54714, Estado de México, Mexico.
  • Ganem-Rondero A; Laboratorio de Investigación y Posgrado en Tecnología Farmacéutica (L-322, Campo 1), Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, 54740, Estado de México, Mexico.
  • Nava-Arzaluz MG; Laboratorio de Investigación y Posgrado en Tecnología Farmacéutica (L-322, Campo 1), Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, 54740, Estado de México, Mexico.
  • Becerril-Osnaya AA; Laboratorio de Microbiología (Anexo L-513, Campo 1), Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, 54740, Cuautitlán Izcalli, Mexico.
  • Pérez-Carranza LA; Laboratorio de Microbiología (Anexo L-513, Campo 1), Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, 54740, Cuautitlán Izcalli, Mexico.
  • Alcalá-Alcalá S; Laboratorio de Tecnología Farmacéutica, Facultad de Farmacia, Universidad Autónoma del Estado de Morelos, Cuernavaca, 62209, México.
  • Mendoza-Muñoz N; Laboratorio de Farmacia, Facultad de Ciencias Químicas, Universidad de Colima, Colima, 28040, México.
  • Piñón-Segundo E; Laboratorio de Sistemas Farmacéuticos de Liberación Modificada (L-13, UIM), Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México, 54714, Estado de México, Mexico. pinonsegundoelizabeth@cuautitlan.unam.mx.
AAPS PharmSciTech ; 25(7): 197, 2024 Aug 22.
Article em En | MEDLINE | ID: mdl-39174702
ABSTRACT
Vulvovaginal candidiasis (VVC) alters the innate cervicovaginal immunity, which provides an important barrier against viruses and other infections. The incidence of this disease has not decreased in the last 30 years, so effective treatments are still needed. Nanoparticles (NPs) of cellulose acetate phthalate (CAP) and clotrimazole (CLZ) were prepared by the emulsification-diffusion method. NPs were characterized using dynamic light scattering, atomic force microscopy and differential scanning calorimetry; their release profile was determined by the dialysis bag technique and mucoadhesion was evaluated with the mucin-particle method. The growth inhibition study of Candida albicans was carried out using the plate counting technique. Finally, accelerated physical stability tests of NPs were carried out, both in water and in SVF. The CAP-CLZ NPs had an average diameter of 273.4 nm, a PDI of 0.284, smooth surfaces and spherical shapes. In vitro release of CLZ from the CAP NPs was categorized with the Weibull model as a matrix system in which initial release was rapid and subsequently sustained. The inhibition of C. albicans growth by the CAP-CLZ NPs was greater than that of free CLZ, and the CAP-only NPs had a microbicidal effect on C. albicans. The NPs showed poor mucoadhesiveness, which could lead to studies of their mucopenetration capacities. An accelerated physical stability test revealed the erosion of CAP in aqueous media. A nanoparticulate system was developed and provided sustained release of CLZ, and it combined an antifungal agent with a microbial polymer that exhibited antifungal activity against C. albicans.
Assuntos
Palavras-chave
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Candida albicans / Candidíase Vulvovaginal / Celulose / Clotrimazol / Nanopartículas / Antifúngicos Idioma: En Revista: AAPS PharmSciTech Assunto da revista: FARMACOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: México País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Candida albicans / Candidíase Vulvovaginal / Celulose / Clotrimazol / Nanopartículas / Antifúngicos Idioma: En Revista: AAPS PharmSciTech Assunto da revista: FARMACOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: México País de publicação: Estados Unidos