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The actin-spectrin submembrane scaffold restricts endocytosis along proximal axons.
Wernert, Florian; Moparthi, Satish Babu; Pelletier, Florence; Lainé, Jeanne; Simons, Eline; Moulay, Gilles; Rueda, Fanny; Jullien, Nicolas; Benkhelifa-Ziyyat, Sofia; Papandréou, Marie-Jeanne; Leterrier, Christophe; Vassilopoulos, Stéphane.
Afiliação
  • Wernert F; Aix Marseille Université, CNRS, INP UMR7051, NeuroCyto, 13005 Marseille, France.
  • Moparthi SB; Sorbonne Université, INSERM, Institute of Myology, Centre of Research in Myology, UMRS 974, Paris, France.
  • Pelletier F; Aix Marseille Université, CNRS, INP UMR7051, NeuroCyto, 13005 Marseille, France.
  • Lainé J; Sorbonne Université, Department of Physiology, Faculty of Medicine Pitié-Salpêtrière, Paris, France.
  • Simons E; Aix Marseille Université, CNRS, INP UMR7051, NeuroCyto, 13005 Marseille, France.
  • Moulay G; Sorbonne Université, INSERM, Institute of Myology, Centre of Research in Myology, UMRS 974, Paris, France.
  • Rueda F; Aix Marseille Université, CNRS, INP UMR7051, NeuroCyto, 13005 Marseille, France.
  • Jullien N; Aix Marseille Université, CNRS, INP UMR7051, NeuroCyto, 13005 Marseille, France.
  • Benkhelifa-Ziyyat S; Sorbonne Université, INSERM, Institute of Myology, Centre of Research in Myology, UMRS 974, Paris, France.
  • Papandréou MJ; Aix Marseille Université, CNRS, INP UMR7051, NeuroCyto, 13005 Marseille, France.
  • Leterrier C; Aix Marseille Université, CNRS, INP UMR7051, NeuroCyto, 13005 Marseille, France.
  • Vassilopoulos S; Sorbonne Université, INSERM, Institute of Myology, Centre of Research in Myology, UMRS 974, Paris, France.
Science ; 385(6711): eado2032, 2024 08 23.
Article em En | MEDLINE | ID: mdl-39172837
ABSTRACT
Clathrin-mediated endocytosis has characteristic features in neuronal dendrites and presynapses, but how membrane proteins are internalized along the axon shaft remains unclear. We focused on clathrin-coated structures and endocytosis along the axon initial segment (AIS) and their relationship to the periodic actin-spectrin scaffold that lines the axonal plasma membrane. A combination of super-resolution microscopy and platinum-replica electron microscopy on cultured neurons revealed that AIS clathrin-coated pits form within "clearings", circular areas devoid of actin-spectrin mesh. Actin-spectrin scaffold disorganization increased clathrin-coated pit formation. Cargo uptake and live-cell imaging showed that AIS clathrin-coated pits are particularly stable. Neuronal plasticity-inducing stimulation triggered internalization of the clathrin-coated pits through polymerization of branched actin around them. Thus, spectrin and actin regulate clathrin-coated pit formation and scission to control endocytosis at the AIS.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Axônios / Actinas / Espectrina / Clatrina / Endocitose Limite: Animals / Humans Idioma: En Revista: Science Ano de publicação: 2024 Tipo de documento: Article País de afiliação: França País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Axônios / Actinas / Espectrina / Clatrina / Endocitose Limite: Animals / Humans Idioma: En Revista: Science Ano de publicação: 2024 Tipo de documento: Article País de afiliação: França País de publicação: Estados Unidos