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Application of Reducible Covalent Capture Purification for Resolving Persulfidome and Nucleolin S-Sulfhydration.
Huang, Wei-Chieh; Hsu, Kai-Wen; Peng, Pei-Hua; Zeng, Wan-Ting; Gu, Ting-Jia; Lin, Li-Jie; Hsieh, Min-Tsang; Lee, Der-Yen; Chang, Geen-Dong.
Afiliação
  • Huang WC; Graduate Institute of Biochemical Sciences, National Taiwan University, No.1, Section 4, Roosevelt Road, Taipei 106, Taiwan.
  • Hsu KW; Drug Development Center, Program for Cancer Biology and Drug Discovery, China Medical University, No. 91, Hsueh-Shih Road, Taichung 40402, Taiwan.
  • Peng PH; Research Center for Cancer Biology, China Medical University, No. 91, Hsueh-Shih Road, Taichung 40402, Taiwan.
  • Zeng WT; Institute of Translational Medicine and New Drug Development, China Medical University, No. 91, Hsueh-Shih Road, Taichung 40402, Taiwan.
  • Gu TJ; Drug Development Center, Program for Cancer Biology and Drug Discovery, China Medical University, No. 91, Hsueh-Shih Road, Taichung 40402, Taiwan.
  • Lin LJ; Graduate Institute of Integrated Medicine, China Medical University, No. 91, Hsueh-Shih Road, Taichung 40402, Taiwan.
  • Hsieh MT; Graduate Institute of Integrated Medicine, China Medical University, No. 91, Hsueh-Shih Road, Taichung 40402, Taiwan.
  • Lee DY; Drug Development Center, Program for Cancer Biology and Drug Discovery, China Medical University, No. 91, Hsueh-Shih Road, Taichung 40402, Taiwan.
  • Chang GD; Research Center for Cancer Biology, China Medical University, No. 91, Hsueh-Shih Road, Taichung 40402, Taiwan.
Anal Chem ; 96(35): 14186-14196, 2024 Sep 03.
Article em En | MEDLINE | ID: mdl-39171919
ABSTRACT
Protein S-sulfhydration involves the regulation of various protein functions, and resolving the S-sulfhydrated proteome (persulfidome) allows for a deeper exploration of various redox regulations. Therefore, we designed a reducible covalent capture method for isolating S-sulfhydrated proteins, which can analyze the persulfidome in biological samples and monitor specific S-sulfhydrated proteins. In this study, we applied this method to reveal the S-sulfhydration levels of proteins, including 3-phosphoglyceraldehyde dehydrogenase, NFκB/p65, and nucleolin. Furthermore, this technique can be used to enrich S-sulfhydrated peptides, aiding in the determination of protein S-sulfhydration modification sites. Finally, we observed that the S-sulfhydration and oxidation of nucleolin on the C543 residue correlate with its nuclear translocation, downstream regulation of p53, Bcl-xL, and Bcl-2 RNA levels and protein expression, as well as the protective function against oxidative stress. Therefore, this method may facilitate the understanding of the regulation of protein function by redox perturbation.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oxirredução / Fosfoproteínas / Proteínas de Ligação a RNA / Nucleolina Limite: Humans Idioma: En Revista: Anal Chem Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Taiwan País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oxirredução / Fosfoproteínas / Proteínas de Ligação a RNA / Nucleolina Limite: Humans Idioma: En Revista: Anal Chem Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Taiwan País de publicação: Estados Unidos