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Oral cancer screening using cytopathology: An advanced proposal with the Bethesda classification.
Lepper, Tatiana Wannmacher; Ferreira Prigol, Renata; Ferrares Espinosa, Ana Laura; Guedes, Igor Cavalcante; Maciel Rönnau, Maikel; Daroit, Natália Batista; Haas, Alex Nogueira; Visioli, Fernanda; Menezes de Oliveira Neto, Manuel; Rados, Pantelis Varvaki.
Afiliação
  • Lepper TW; Dental School - Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
  • Ferreira Prigol R; Dental School - Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
  • Ferrares Espinosa AL; Dental School - Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
  • Guedes IC; Dental School - Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
  • Maciel Rönnau M; Informatics Institute - Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
  • Daroit NB; Dental School - Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
  • Haas AN; Dental School - Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
  • Visioli F; Dental School - Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
  • Menezes de Oliveira Neto M; Informatics Institute - Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
  • Rados PV; Dental School - Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Cytopathology ; 2024 Aug 20.
Article em En | MEDLINE | ID: mdl-39164925
ABSTRACT

BACKGROUND:

Oral cancer screening requires analysis capable of detecting changes preceding the clinical manifestation. Oral cytopathology studies as an oral cancer screening have shown promising results. This study aims to evaluate the use of cytopathology in a quali-quantitative analysis using Papanicoloau staining.

METHODS:

Four experimental groups were evaluated control (CG), exposed to carcinogens (EG), potentially malignant disorder with and without epithelial dysplasia (D-OPMDG and ND-OPMDG) and oral squamous cell carcinoma (OSCCG). Oral smears were collected using a Cytobrush® and conventional exfoliative cytology.

RESULTS:

Oral Papanicolaou smears from 143 individuals were analysed in 8100 images. Qualitatively, non-lesional groups exhibited minimal suspected cases (20% in CG and 5% in EG), in the OPMD groups the ability to identify altered cells was low (40% in D-OPMDG and 0% in ND-OPMDG). Conversely, a notable 100% accuracy was achieved in the OSCCG. Quantitatively, a higher percentage of anucleated and a lower percentage of intermediate cells were observed in the OPMDG; a higher proportion of parabasal/suspicious cells was observed in OSCCG. The optimal threshold for improved accuracy in detecting suspected malignancies occurs when the count of parabasal/suspicious cells exceeds 8/100 cells examined. Cytomorphometric analysis revealed a higher nucleus/cytoplasm ratio (N/C) in OSCCG, with a best cutoff point indicating enhanced accuracy in discerning suspected malignancies when exceeding 17% of cells exhibiting this elevated ratio.

CONCLUSION:

It is possible to suggest updates in cytomorphometric and quantitative analysis in the modified Bethesda system for the oral cavity including objective criteria in the risk classification, therefore improving oral cancer screening.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Cytopathology Assunto da revista: PATOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Cytopathology Assunto da revista: PATOLOGIA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido