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Antioxidant activity of Zuccagnia-type propolis: A combined approach based on LC-HRMS analysis of bioanalytical-guided fractions and computational investigation.
Solorzano, Eliana Rita; Roverso, Marco; Bogialli, Sara; Bortoli, Marco; Orian, Laura; Badocco, Denis; Pettenuzzo, Silvia; Favaro, Gabriella; Pastore, Paolo.
Afiliação
  • Solorzano ER; Department of Chemical Sciences, University of Padua, Via Marzolo, 1, 35131 Padova, Italy; Facultad de Bioquimica, Quimica y Farmacia, Instituto de Fisica, Universidad Nacional de Tucuman, Argentina.
  • Roverso M; Department of Chemical Sciences, University of Padua, Via Marzolo, 1, 35131 Padova, Italy.
  • Bogialli S; Department of Chemical Sciences, University of Padua, Via Marzolo, 1, 35131 Padova, Italy. Electronic address: sara.bogialli@unipd.it.
  • Bortoli M; Department of Chemical Sciences, University of Padua, Via Marzolo, 1, 35131 Padova, Italy; Hylleraas Centre for Quantum Molecular Sciences and Department of Chemistry, University of Oslo, PO Box 1033 Blindern, 0315 Oslo, Norway.
  • Orian L; Department of Chemical Sciences, University of Padua, Via Marzolo, 1, 35131 Padova, Italy.
  • Badocco D; Department of Chemical Sciences, University of Padua, Via Marzolo, 1, 35131 Padova, Italy.
  • Pettenuzzo S; Department of Chemical Sciences, University of Padua, Via Marzolo, 1, 35131 Padova, Italy; Department Civil, Environmental and Architectural Engineering, University of Padua, Via Marzolo, 9, 35131, Padova, Italy.
  • Favaro G; Department of Chemical Sciences, University of Padua, Via Marzolo, 1, 35131 Padova, Italy.
  • Pastore P; Department of Chemical Sciences, University of Padua, Via Marzolo, 1, 35131 Padova, Italy.
Food Chem ; 461: 140827, 2024 Dec 15.
Article em En | MEDLINE | ID: mdl-39146684
ABSTRACT
This study reports a combined approach to assess the antioxidant activity of Zuccagnia-type propolis. Fractions exhibiting the highest antioxidant activities evidenced by DPPH, a ß-carotene bleaching and superoxide radical scavenging activity-non-enzymatic assays, were processed by LC-HRMS/MS to characterize the relevant chemical compounds. A computational protocol based on the DFT calculations was used to rationalize the main outcomes. Among the 28 identified flavonoids, caffeic acids derivatives were in the fraction exhibiting the highest antioxidant activity, with 1-methyl-3-(4'-hydroxyphenyl)-propyl caffeic acid ester and 1-methyl-3-(3',4'-dihydroxyphenyl)-propyl caffeic acid ester as major components. Results clearly showed roles of specific chemical motifs, which can be supported by the computational analysis. This is the first report ascribing the antioxidant ability of Zuccagnia-type propolis to its content in specific caffeic acid derivatives, a potential source of radical scavenging phytochemicals. The proposed protocol can be extended to the study of other plant-products to address the most interesting bioactive compounds.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Própole / Espectrometria de Massas em Tandem / Antioxidantes Idioma: En Revista: Food Chem Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Argentina País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Própole / Espectrometria de Massas em Tandem / Antioxidantes Idioma: En Revista: Food Chem Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Argentina País de publicação: Reino Unido