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Pirfenidone improves early cardiac function following myocardial infarction by enhancing the elastin/collagen ratio.
Yu, Yuexin; Xu, Yaping; Chen, Jinfu; Yao, Yao; Liu, Yingtian; Chen, Yan; Yang, Bin; Guo, Zhikun.
Afiliação
  • Yu Y; Henan Key Laboratory of Cardiac Remodeling and Transplantation, Zhengzhou Seventh People's Hospital, Zhengzhou, Henan 450016, China; Henan Key Laboratory of Medical Tissue Regeneration, Xinxiang Medical University, Xinxiang, Henan 453003, China. Electronic address: 544892985@qq.com.
  • Xu Y; Henan Key Laboratory of Cardiac Remodeling and Transplantation, Zhengzhou Seventh People's Hospital, Zhengzhou, Henan 450016, China; USM-ALPS Cardiac Research Laboratory, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Penang 13200, Malaysia. Electronic address: xuyaping@student.us
  • Chen J; Henan Key Laboratory of Cardiac Remodeling and Transplantation, Zhengzhou Seventh People's Hospital, Zhengzhou, Henan 450016, China. Electronic address: 402875646@qq.com.
  • Yao Y; Henan Key Laboratory of Medical Tissue Regeneration, Xinxiang Medical University, Xinxiang, Henan 453003, China. Electronic address: 64996253@qq.com.
  • Liu Y; Henan Key Laboratory of Medical Tissue Regeneration, Xinxiang Medical University, Xinxiang, Henan 453003, China. Electronic address: 1123368560@qq.com.
  • Chen Y; Henan Key Laboratory of Medical Tissue Regeneration, Xinxiang Medical University, Xinxiang, Henan 453003, China. Electronic address: 448202359@qq.com.
  • Yang B; Henan Key Laboratory of Cardiac Remodeling and Transplantation, Zhengzhou Seventh People's Hospital, Zhengzhou, Henan 450016, China. Electronic address: yangbin166@163.com.
  • Guo Z; Henan Key Laboratory of Cardiac Remodeling and Transplantation, Zhengzhou Seventh People's Hospital, Zhengzhou, Henan 450016, China; Henan Key Laboratory of Medical Tissue Regeneration, Xinxiang Medical University, Xinxiang, Henan 453003, China. Electronic address: gzk@xxmu.edu.cn.
Biomed Pharmacother ; 178: 117254, 2024 Sep.
Article em En | MEDLINE | ID: mdl-39142250
ABSTRACT

BACKGROUND:

Acute myocardial infarction (AMI) is a leading cause of mortality worldwide, with reduced elastin/collagen ratios exacerbating cardiac dysfunction due to collagen-rich scar tissue replacing necrotic myocardial cells. This study aims to evaluate pirfenidone's therapeutic effect on early cardiac function post-AMI and elucidate its impact on the elastin/collagen ratio.

METHODS:

Sprague-Dawley rats were divided into four groups Sham, AMI, AMI treated with PBS (AMI-PBS), and AMI treated with pirfenidone (AMI-PFD) (n=12 each). AMI was induced via coronary artery ligation. The AMI-PFD and AMI-PBS groups received pirfenidone and PBS for 14 days, respectively. Cardiac function, fibrosis, serum cytokines, collagen and elastin content, and their ratios were assessed. Cardiac fibroblasts (CFs) from neonatal rats were categorized into control, hypoxia-induced (LO), LO+PBS, and LO+PFD groups. ELISA measured inflammatory factors, and RT-PCR analyzed collagen and elastin gene expression.

RESULTS:

The AMI-PFD group showed improved cardiac function and reduced serum interleukin-1ß (IL-1ß), IL-6, and transforming growth factor-ß (TGF-ß). Type I and III collagen decreased by 22.6 % (P=0.0441) and 34.4 % (P=0.0427), respectively, while elastin content increased by 79.4 % (P=0.0126). E/COLI and E/COLIII ratios rose by 81.1 % (P=0.0026) and 88.1 % (P=0.0006). CFs in the LO+PFD group exhibited decreased IL-1ß, IL-6, TGF-ß, type I and III collagen, with increased elastin mRNA, enhancing the elastin/collagen ratio.

CONCLUSION:

Pirfenidone enhances cardiac function by augmenting the early elastin/collagen ratio post-AMI.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Piridonas / Colágeno / Elastina / Infarto do Miocárdio Limite: Animals Idioma: En Revista: Biomed Pharmacother Ano de publicação: 2024 Tipo de documento: Article País de publicação: França
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Piridonas / Colágeno / Elastina / Infarto do Miocárdio Limite: Animals Idioma: En Revista: Biomed Pharmacother Ano de publicação: 2024 Tipo de documento: Article País de publicação: França