Characterization of <i>GPX</i> Gene Family in Pepper (<i>Capsicum annuum</i> L.) under Abiotic Stress and ABA Treatment.

Zhang, Zeyu; Zhang, Jing; Wang, Cheng; Chang, Youlin; Han, Kangning; Gao, Yanqiang; Xie, Jianming

Characterization of GPX Gene Family in Pepper (Capsicum annuum L.) under Abiotic Stress and ABA Treatment.

Zhang, Zeyu; Zhang, Jing; Wang, Cheng; Chang, Youlin; Han, Kangning; Gao, Yanqiang; Xie, Jianming.

Afiliação

Zhang Z; College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China.
Zhang J; College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China.
Wang C; College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China.
Chang Y; College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China.
Han K; College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China.
Gao Y; College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China.
Xie J; College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China.

Int J Mol Sci ; 25(15)2024 Jul 30.

Article em En | MEDLINE | ID: mdl-39125911

ABSTRACT

ABSTRACT

Plant glutathione peroxidases (GPXs) are important enzymes for removing reactive oxygen species in plant cells and are closely related to the stress resistance of plants. This study identified the GPX gene family members of pepper (Capsicum annuum L.), "CM333", at the whole-genome level to clarify their expression patterns and enzyme activity changes under abiotic stress and ABA treatment. The results showed that eight CaGPX genes were unevenly distributed across four chromosomes and one scaffold of the pepper genome, and their protein sequences had Cys residues typical of the plant GPX domains. The analysis of collinearity, phylogenetic tree, gene structure, and conserved motifs indicated that the CaGPX gene sequence is conserved, structurally similar, and more closely related to the sequence structure of Arabidopsis. Meanwhile, many cis elements involved in stress, hormones, development, and light response were found in the promoter region of the CaGPX gene. In addition, CaGPX1/4 and CaGPX6 were basically expressed in all tissues, and their expression levels were significantly upregulated under abiotic stress and ABA treatment. Subcellular localization showed that CaGPX1 and CaGPX4 are localized in chloroplasts. Additionally, the variations in glutathione peroxidase activity (GSH-Px) mostly agreed with the variations in gene expression. In summary, the CaGPXs gene may play an important role in the development of peppers and their response to abiotic stress and hormones.

Assuntos

Ácido Abscísico; Capsicum; Regulação da Expressão Gênica de Plantas; Glutationa Peroxidase; Família Multigênica; Filogenia; Proteínas de Plantas; Estresse Fisiológico; Capsicum/genética; Capsicum/enzimologia; Capsicum/efeitos dos fármacos; Regulação da Expressão Gênica de Plantas/efeitos dos fármacos; Estresse Fisiológico/genética; Ácido Abscísico/farmacologia; Ácido Abscísico/metabolismo; Glutationa Peroxidase/genética; Glutationa Peroxidase/metabolismo; Proteínas de Plantas/genética; Proteínas de Plantas/metabolismo; Regiões Promotoras Genéticas; Sequência de Aminoácidos

Palavras-chave

abiotic stress; expression pattern; glutathione peroxidase; pepper

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Filogenia / Proteínas de Plantas / Estresse Fisiológico / Capsicum / Família Multigênica / Ácido Abscísico / Regulação da Expressão Gênica de Plantas / Glutationa Peroxidase Idioma: En Revista: Int J Mol Sci Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China País de publicação: Suíça

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