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Synthesis of Keratin Nanoparticles Extracted from Human Hair through Hydrolysis with Concentrated Sulfuric Acid: Characterization and Cytotoxicity.
Silva, Otavio A; Rossin, Ariane R S; Lima, Antônia M de Oliveira; Valente, Andressa D; Garcia, Francielle P; Nakamura, Celso V; Follmann, Heveline D M; Silva, Rafael; Martins, Alessandro F.
Afiliação
  • Silva OA; Department of Chemistry, State University of Maringa, Maringa 87020-900, PR, Brazil.
  • Rossin ARS; Department of Chemistry, State University of Maringa, Maringa 87020-900, PR, Brazil.
  • Lima AMO; Postgraduate Program in Chemistry, State University of West Paraná, Toledo 85903-000, PR, Brazil.
  • Valente AD; Department of Chemistry, State University of Maringa, Maringa 87020-900, PR, Brazil.
  • Garcia FP; Research Laboratory, Federal Institute of Maranhão-Imperatriz, Imperatriz 65900-000, MA, Brazil.
  • Nakamura CV; Department of Basic Health Sciences, State University of Maringa, Maringa 87020-900, PR, Brazil.
  • Follmann HDM; Department of Basic Health Sciences, State University of Maringa, Maringa 87020-900, PR, Brazil.
  • Silva R; Department of Basic Health Sciences, State University of Maringa, Maringa 87020-900, PR, Brazil.
  • Martins AF; Department of Chemistry, State University of Maringa, Maringa 87020-900, PR, Brazil.
Materials (Basel) ; 17(15)2024 Jul 30.
Article em En | MEDLINE | ID: mdl-39124423
ABSTRACT
Human hair, composed primarily of keratin, represents a sustainable waste material suitable for various applications. Synthesizing keratin nanoparticles (KNPs) from human hair for biomedical uses is particularly attractive due to their biocompatibility. In this study, keratin was extracted from human hair using concentrated sulfuric acid as the hydrolysis agent for the first time. This process yielded KNPs in both the supernatant (KNPs-S) and precipitate (KNPs-P) phases. Characterization involved scanning electron microscopy (SEM), Fourier-transform infrared spectroscopy (FTIR), Zeta potential analysis, X-ray diffraction (XRD), and thermogravimetric analysis (TG). KNPs-S and KNPs-P exhibited average diameters of 72 ± 5 nm and 27 ± 5 nm, respectively. The hydrolysis process induced a structural rearrangement favoring ß-sheet structures over α-helices in the KNPs. These nanoparticles demonstrated negative Zeta potentials across the pH spectrum. KNPs-S showed higher cytotoxicity (CC50 = 176.67 µg/mL) and hemolytic activity, likely due to their smaller size compared to KNPs-P (CC50 = 246.21 µg/mL), particularly at concentrations of 500 and 1000 µg/mL. In contrast, KNPs-P did not exhibit hemolytic activity within the tested concentration range of 32.5 to 1000 µg/mL. Both KNPs demonstrated cytocompatibility with fibroblast cells in a dose-dependent manner. Compared to other methods reported in the literature and despite requiring careful washing and neutralization steps, sulfuric acid hydrolysis proved effective, rapid, and feasible for producing cytocompatible KNPs (biomaterials) in single-step synthesis.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Materials (Basel) Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Materials (Basel) Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça