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Chitosan-coated manganese ferrite nanoparticles enhanced Rhodotorula toruloides carotenoid production.
Ochoa-Viñals, Nayra; Alonso-Estrada, Dania; Ramos-González, Rodolfo; Rodríguez-Hernández, Joelis; Martínez-Hernández, José Luis; Aguilar-González, Miguel Ángel; Betancourt-Galindo, Rebeca; Michelena-Álvarez, Georgina Lourdes; Ilina, Anna.
Afiliação
  • Ochoa-Viñals N; Facultad de Ciencias Químicas, Universidad Autónoma de Coahuila, Unidad Saltillo, 25280, Saltillo, Coahuila, Mexico.
  • Alonso-Estrada D; Facultad de Ciencias Químicas, Universidad Autónoma de Coahuila, Unidad Saltillo, 25280, Saltillo, Coahuila, Mexico.
  • Ramos-González R; CONAHCYT, Universidad Autónoma de Coahuila, Unidad Saltillo, 25280, Saltillo, Coahuila, Mexico. rodolfo.ramos@uadec.edu.mx.
  • Rodríguez-Hernández J; Centro de Investigación en Química Aplicada, Blvd. Enrique Reyna Hermosillo No. 140, 25250, Saltillo, Coahuila, Mexico.
  • Martínez-Hernández JL; Facultad de Ciencias Químicas, Universidad Autónoma de Coahuila, Unidad Saltillo, 25280, Saltillo, Coahuila, Mexico.
  • Aguilar-González MÁ; Facultad de Ciencias Químicas, Universidad Autónoma de Coahuila, Unidad Saltillo, 25280, Saltillo, Coahuila, Mexico.
  • Betancourt-Galindo R; Centro de Investigación en Química Aplicada, Blvd. Enrique Reyna Hermosillo No. 140, 25250, Saltillo, Coahuila, Mexico.
  • Michelena-Álvarez GL; Instituto Cubano de Investigaciones de Los Derivados de La Caña de Azúcar, San Miguel Del Padrón, 11000, La Habana, Cuba.
  • Ilina A; Facultad de Ciencias Químicas, Universidad Autónoma de Coahuila, Unidad Saltillo, 25280, Saltillo, Coahuila, Mexico. annailina@uadec.edu.mx.
Bioprocess Biosyst Eng ; 47(11): 1777-1787, 2024 Nov.
Article em En | MEDLINE | ID: mdl-39090227
ABSTRACT
The present study aims to analyze the interaction between Rhodotorula toruloides and magnetic nanoparticles and evaluate their effect on carotenoid production. The manganese ferrite nanoparticles were synthesized without chitosan (MnFe2O4) and chitosan coating (MnFe2O4-CS) by the co-precipitation method assisted by hydrothermal treatment. XRD (X-ray diffraction), Magnetometry, Dynamic Light Scattering (DLS) and FTIR (Fourier-Transform Infrared Spectroscopy), are used to characterize the magnetic nanoparticles. The crystallite size of MnFe2O4 was 16 nm for MnFe2O4 and 20 nm for MnFe2O4-CS. The magnetic saturation of MnFe2O4-CS was lower (39.6 ± 0.6 emu/g) than the same MnFe2O4 nanoparticles (42.7 ± 0.3 emu/g), which was attributed to the chitosan fraction presence. The MnFe2O4-CS FTIR spectra revealed the presence of the characteristic chitosan bands. DLS demonstrated that the average hydrodynamic diameters were 344 nm for MnFe2O4 and 167 nm for MnFe2O4-CS. A kinetic study of cell immobilization performed with their precipitation with a magnet demonstrated that interaction between magnetic nanoparticles and R. toruloides was characterized by an equilibrium time of 2 h. The adsorption isotherm models (Langmuir and Freundlich) were fitted to the experimental values. The trypan blue assay was used for cell viability assessment. The carotenoid production increased to 256.2 ± 6.1 µg/g dry mass at 2.0 mg/mL MnFe2O4-CS. The use of MnFe2O4-CS to stimulate carotenoid yeast production and the magnetic separation of biomass are promising nanobiotechnological alternatives. Magnetic cell immobilization is a perspective technique for obtaining cell metabolites.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Rhodotorula / Compostos Férricos / Carotenoides / Compostos de Manganês / Quitosana Idioma: En Revista: Bioprocess Biosyst Eng Assunto da revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: México País de publicação: Alemanha
Texto completo
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PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Rhodotorula / Compostos Férricos / Carotenoides / Compostos de Manganês / Quitosana Idioma: En Revista: Bioprocess Biosyst Eng Assunto da revista: BIOTECNOLOGIA / ENGENHARIA BIOMEDICA Ano de publicação: 2024 Tipo de documento: Article País de afiliação: México País de publicação: Alemanha