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In vitro probiotic potential of lactic acid bacteria isolated from the intestines of Muscovy ducks.
de Carvalho Maquiné, Leandro; Dos Santos Almeida Coelho, Kelven Wladie; da Silva Gomes, Maria Fernanda; Vieira, Jackelyne Ribamar; Cavalcante, Thatiana Farias; de Souza Carvalho, Elen Bethleen; Rufino, João Paulo Ferreira; de Oliveira, Adriano Teixeira; de Queiroz Costa Neto, Pedro; Pereira, José Odair.
Afiliação
  • de Carvalho Maquiné L; Faculty of Agrarian Sciences, Federal University of Amazonas, Manaus, Amazonas, Brazil.
  • Dos Santos Almeida Coelho KW; Faculty of Agrarian Sciences, Federal University of Amazonas, Manaus, Amazonas, Brazil.
  • da Silva Gomes MF; Faculty of Agrarian Sciences, Federal University of Amazonas, Manaus, Amazonas, Brazil.
  • Vieira JR; Institute of Biological Sciences, Federal University of Amazonas, Manaus, Amazonas, Brazil.
  • Cavalcante TF; Institute of Biological Sciences, Federal University of Amazonas, Manaus, Amazonas, Brazil.
  • de Souza Carvalho EB; Institute of Biological Sciences, Federal University of Amazonas, Manaus, Amazonas, Brazil.
  • Rufino JPF; Faculty of Agrarian Sciences, Federal University of Amazonas, Manaus, Amazonas, Brazil. joaopaulorufino@live.com.
  • de Oliveira AT; Science, and Technology of Amazonas - Center Campus of Manaus, Federal Institute of Education, Manaus City, Amazonas State, Brazil.
  • de Queiroz Costa Neto P; Faculty of Agrarian Sciences, Federal University of Amazonas, Manaus, Amazonas, Brazil.
  • Pereira JO; Faculty of Agrarian Sciences, Federal University of Amazonas, Manaus, Amazonas, Brazil.
Braz J Microbiol ; 2024 Jul 30.
Article em En | MEDLINE | ID: mdl-39080108
ABSTRACT
The current study was conducted to isolate, test and characterize molecularly and physiologically lactic acid bacteria from the intestines of Muscovy ducks to evaluate their probiotic potential for poultry farming. Three hundred lactic acid bacteria from the gastrointestinal tract of Muscovy ducks were isolated. The strains were phenotypically characterized by observing cell morphology, performing Gram staining, catalase production, and testing their ability to grow in MRS broth at different temperatures, pH values, NaCl concentrations, bile concentration, and in compatibility tests between strains. Nine strains were selected based on their resilience. Eight strains were identified using molecular techniques. These strains exhibited significant tolerance to acidic pH, bile salts, and NaCl, essential for probiotic function. All isolates inhibited the growth of Salmonella enterica serotype Typhimurium (DT104) and Enteropathogenic Escherichia coli serotype O86H34 (EPEC), showcasing their antimicrobial potential. Antibiotic susceptibility testing revealed 100% resistance to clindamycin and erythromycin but high susceptibility to ampicillin and vancomycin. Growth was observed at various temperatures, indicating mesophilic characteristics. Compatibility tests confirmed their suitability for probiotic formulations. Genomic analysis identified the strains primarily as Enterococcus. Conclusively, the study identified eight out of nine selected lactic acid bacteria strains from Muscovy ducks as autochthonous probiotics, showing resilience to treatments and compatibility for consortium formulation. These strains are suitable for in vivo testing for potential poultry farming applications. Further research on their molecular mechanisms and in vivo effects is needed.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Braz J Microbiol Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Braz J Microbiol Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Brasil