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Lactate-carried Mitochondrial Energy Overflow.
Rauseo, Daniela; Contreras-Baeza, Yasna; Faurand, Hugo; Cárcamo, Nataly; Suárez, Raibel; von Faber-Castell, Alexandra; Silva, Franco; Mora-González, Valentina; Wyss, Matthias T; Baeza-Lehnert, Felipe; Ruminot, Iván; Alvarez-Navarro, Carlos; San Martín, Alejandro; Weber, Bruno; Sandoval, Pamela Y; Barros, L Felipe.
Afiliação
  • Rauseo D; Centro de Estudios Científicos-CECs, Valdivia, Chile.
  • Contreras-Baeza Y; Facultad de Medicina y Ciencia, Universidad San Sebastián, Valdivia, Chile.
  • Faurand H; Universidad Austral de Chile, Valdivia, Chile.
  • Cárcamo N; Centro de Estudios Científicos-CECs, Valdivia, Chile.
  • Suárez R; Facultad de Medicina y Ciencia, Universidad San Sebastián, Valdivia, Chile.
  • von Faber-Castell A; Universidad Austral de Chile, Valdivia, Chile.
  • Silva F; Centro de Estudios Científicos-CECs, Valdivia, Chile.
  • Mora-González V; Facultad de Medicina y Ciencia, Universidad San Sebastián, Valdivia, Chile.
  • Wyss MT; Centro de Estudios Científicos-CECs, Valdivia, Chile.
  • Baeza-Lehnert F; Facultad de Ciencias para el Cuidado de la Salud, Universidad San Sebastián, Valdivia, Chile.
  • Ruminot I; Centro de Estudios Científicos-CECs, Valdivia, Chile.
  • Alvarez-Navarro C; Facultad de Medicina y Ciencia, Universidad San Sebastián, Valdivia, Chile.
  • San Martín A; Institute of Pharmacology and Toxicology, University and ETH Zurich, Switzerland.
  • Weber B; Neuroscience Center Zurich, ETH and University Zurich, Switzerland.
  • Sandoval PY; Centro de Estudios Científicos-CECs, Valdivia, Chile.
  • Barros LF; Facultad de Ciencias para el Cuidado de la Salud, Universidad San Sebastián, Valdivia, Chile.
bioRxiv ; 2024 Jul 20.
Article em En | MEDLINE | ID: mdl-39071354
ABSTRACT
We addressed the question of mitochondrial lactate metabolism using genetically-encoded sensors. The organelle was found to contain a dynamic lactate pool that leads to dose- and time-dependent protein lactylation. In neurons, mitochondrial lactate reported blood lactate levels with high fidelity. The exchange of lactate across the inner mitochondrial membrane was found to be mediated by a high affinity H+-coupled transport system involving the mitochondrial pyruvate carrier MPC. Assessment of electron transport chain activity and determination of lactate flux showed that mitochondria are tonic lactate producers, a phenomenon driven by energization and stimulated by hypoxia. We conclude that an overflow mechanism caps the redox level of mitochondria, while saving energy in the form of lactate.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: BioRxiv Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Chile País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: BioRxiv Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Chile País de publicação: Estados Unidos