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Efferocytosis assay to quantify the engulfment and acidification of apoptotic cells by macrophages using flow cytometry.
Wu, Xun; Wang, Ziyi; Shern, Tyler; Zhang, Hanrui.
Afiliação
  • Wu X; Cardiometabolic Genomics Program, Division of Cardiology, Department of Medicine, Columbia University Irving Medical Center, New York, NY, USA. Electronic address: xw2698@cumc.columbia.edu.
  • Wang Z; Cardiometabolic Genomics Program, Division of Cardiology, Department of Medicine, Columbia University Irving Medical Center, New York, NY, USA.
  • Shern T; Cardiometabolic Genomics Program, Division of Cardiology, Department of Medicine, Columbia University Irving Medical Center, New York, NY, USA.
  • Zhang H; Cardiometabolic Genomics Program, Division of Cardiology, Department of Medicine, Columbia University Irving Medical Center, New York, NY, USA. Electronic address: hz2418@cumc.columbia.edu.
STAR Protoc ; 5(3): 103215, 2024 Sep 20.
Article em En | MEDLINE | ID: mdl-39068649
ABSTRACT
Efficient macrophage efferocytosis maintains homeostasis and resolves inflammation. Here, we provide a protocol to assess the engulfment and acidification of apoptotic cells (ACs) by macrophages. We describe steps for preparing bone marrow-derived macrophages (BMDMs) and peritoneal macrophages (PMs), fluorescent labeling of ACs using both a pH-sensitive dye, pHrodo-Red succinimidyl ester, and a pH-insensitive dye, Hoechst, and subsequent incubation with macrophages for efferocytosis. We then detail procedures for flow cytometry-based quantification of engulfment and acidification. For complete details on the use and execution of this protocol, please refer to Shi and Wu et al.1.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fagocitose / Apoptose / Citometria de Fluxo / Macrófagos Limite: Animals Idioma: En Revista: STAR Protoc Ano de publicação: 2024 Tipo de documento: Article País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fagocitose / Apoptose / Citometria de Fluxo / Macrófagos Limite: Animals Idioma: En Revista: STAR Protoc Ano de publicação: 2024 Tipo de documento: Article País de publicação: Estados Unidos