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The Fluorescent Cell Line SW620-GFP Is a Valuable Model to Monitor Magnetic Hyperthermia.
Rosales, Saray; Hernández-Gutiérrez, Rodolfo; Oaxaca, Alma; López, Zaira; Casillas, Norberto; Knauth, Peter; Quintero, Luis H; Paz, José A; Cholico, Francisco; Velásquez, Celso; Cano, Mario E.
Afiliação
  • Rosales S; Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara, Marcelino García Barragan 1421, Guadalajara 44430, Jalisco, Mexico.
  • Hernández-Gutiérrez R; Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C., Av. Normalistas 800, Guadalajara 44270, Jalisco, Mexico.
  • Oaxaca A; Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C., Av. Normalistas 800, Guadalajara 44270, Jalisco, Mexico.
  • López Z; Centro Universitario de la Ciénega, Universidad de Guadalajara, Avenida Universidad 1115, Ocotlan 47810, Jalisco, Mexico.
  • Casillas N; Centro Universitario de Ciencias Exactas e Ingenierías, Universidad de Guadalajara, Marcelino García Barragan 1421, Guadalajara 44430, Jalisco, Mexico.
  • Knauth P; Centro Universitario de la Ciénega, Universidad de Guadalajara, Avenida Universidad 1115, Ocotlan 47810, Jalisco, Mexico.
  • Quintero LH; Centro Universitario de Ciencias Económico Administrativas, Universidad de Guadalajara, Periférico Norte 799, Col. Los Belenes, Zapopan 45100, Jalisco, Mexico.
  • Paz JA; Centro Universitario de la Ciénega, Universidad de Guadalajara, Avenida Universidad 1115, Ocotlan 47810, Jalisco, Mexico.
  • Cholico F; Centro Universitario de la Ciénega, Universidad de Guadalajara, Avenida Universidad 1115, Ocotlan 47810, Jalisco, Mexico.
  • Velásquez C; Centro Universitario de los Valles, Universidad de Guadalajara, Carretera Guadalajara-Ameca Km. 45.5, Ameca 46600, Jalisco, Mexico.
  • Cano ME; Centro Universitario de la Ciénega, Universidad de Guadalajara, Avenida Universidad 1115, Ocotlan 47810, Jalisco, Mexico.
Bioengineering (Basel) ; 11(7)2024 Jun 21.
Article em En | MEDLINE | ID: mdl-39061720
ABSTRACT
In this work, the cell line SW620-GFP has been used in a complete magnetic hyperthermia assay, from the preparation of the ferrofluid with folate-coated iron oxide nanoparticles to in vivo experiments. The physical and chemical characterization of the nanoparticles evidenced their superparamagnetic behaviour, an average diameter of 12 ± 4 nm, a 2 nm coat thickness, and a high-power loss density. The main innovation of the work is the exclusive capability of viable SW620-GFP cells to emit fluorescence, enabling fast analysis of both, cell viability in vitro with an epifluorescence microscope and tumour size and shape in vivo in a non-invasive manner using the iBox technology. Moreover, with this imaging technique, it was possible to demonstrate the successful tumour size reduction in mice applying magnetic hyperthermia three times a week over 3 weeks.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Bioengineering (Basel) Ano de publicação: 2024 Tipo de documento: Article País de afiliação: México País de publicação: Suíça
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Bioengineering (Basel) Ano de publicação: 2024 Tipo de documento: Article País de afiliação: México País de publicação: Suíça