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Enhanced fatty acid biosynthesis by Sigma28 in stringent responses contributes to multidrug resistance and biofilm formation in Helicobacter pylori.
Xue, Junyuan; Li, Shutong; Wang, Liyuan; Zhao, Yican; Zhang, Lu; Zheng, Yantong; Zhang, Wenxin; Chen, Zhenghong; Jiang, Ting; Sun, Yundong.
Afiliação
  • Xue J; Key Laboratory for Experimental Teratology of the Ministry of Education and Department of Microbiology, School of Basic Medical Science, Shandong University, Jinan, Shandong, China.
  • Li S; Section of Infection and Immunity, Herman Ostrow School of Dentistry, Norris Comprehensive Cancer Center, University of Southern California, Los Angeles, California, USA.
  • Wang L; Key Laboratory for Experimental Teratology of the Ministry of Education and Department of Microbiology, School of Basic Medical Science, Shandong University, Jinan, Shandong, China.
  • Zhao Y; Key Laboratory for Experimental Teratology of the Ministry of Education and Department of Microbiology, School of Basic Medical Science, Shandong University, Jinan, Shandong, China.
  • Zhang L; Key Laboratory for Experimental Teratology of the Ministry of Education and Department of Microbiology, School of Basic Medical Science, Shandong University, Jinan, Shandong, China.
  • Zheng Y; Key Laboratory for Experimental Teratology of the Ministry of Education and Department of Microbiology, School of Basic Medical Science, Shandong University, Jinan, Shandong, China.
  • Zhang W; Key Laboratory for Experimental Teratology of the Ministry of Education and Department of Microbiology, School of Basic Medical Science, Shandong University, Jinan, Shandong, China.
  • Chen Z; Key Laboratory of Microbiology and Parasitology of Education Department of Guizhou, Guizhou Medical University, Guiyang, China.
  • Jiang T; Jiangsu Luye Diagnostic Technology, Wuxi, China.
  • Sun Y; Key Laboratory for Experimental Teratology of the Ministry of Education and Department of Microbiology, School of Basic Medical Science, Shandong University, Jinan, Shandong, China.
Antimicrob Agents Chemother ; 68(9): e0085024, 2024 Sep 04.
Article em En | MEDLINE | ID: mdl-39046242
ABSTRACT
The metabolic state of bacteria significantly contributes to their resistance to antibiotics; however, the specific metabolic mechanisms conferring antimicrobial resistance in Helicobacter pylori remain largely understudied. Employing transcriptomic and non-targeted metabolomics, we characterized the metabolic reprogramming of H. pylori when challenged with antibiotic agents. We observed a notable increase in both genetic and key proteomic components involved in fatty acid biosynthesis. Inhibition of this pathway significantly enhanced the antibiotic susceptibility of the sensitive and multidrug-resistant H. pylori strains while also disrupting their biofilm-forming capacities. Further analysis revealed that antibiotic treatment induced a stringent response, triggering the expression of the hp0560-hp0557 operon regulated by Sigma28 (σ28). This activation in turn stimulated the fatty acid biosynthetic pathway, thereby enhancing the antibiotic tolerance of H. pylori. Our findings reveal a novel adaptive strategy employed by H. pylori to withstand antibiotic stress.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Helicobacter pylori / Biofilmes / Farmacorresistência Bacteriana Múltipla / Ácidos Graxos / Antibacterianos Idioma: En Revista: Antimicrob Agents Chemother Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China País de publicação: Estados Unidos
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Bactérias / Helicobacter pylori / Biofilmes / Farmacorresistência Bacteriana Múltipla / Ácidos Graxos / Antibacterianos Idioma: En Revista: Antimicrob Agents Chemother Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China País de publicação: Estados Unidos