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Impact of acupuncture on ischemia/reperfusion injury: Unraveling the role of miR-34c-5p and autophagy activation.
Lu, Xiao-Ye; Lv, Qian-Yi; Li, Qi-Long; Zhang, Hong; Chen, Chu-Tao; Tian, Hao-Mei.
Afiliação
  • Lu XY; College of Acupuncture and Tuina and Rehabilitation, Hunan University of Traditional Chinese Medicine, Changsha, Hunan Province 410007, China; Department of Rehabilitation, Changsha Central Hospital, Changsha, Hunan Province 410004, China.
  • Lv QY; College of Acupuncture and Tuina and Rehabilitation, Hunan University of Traditional Chinese Medicine, Changsha, Hunan Province 410007, China.
  • Li QL; College of Acupuncture and Tuina and Rehabilitation, Hunan University of Traditional Chinese Medicine, Changsha, Hunan Province 410007, China.
  • Zhang H; College of Acupuncture and Tuina and Rehabilitation, Hunan University of Traditional Chinese Medicine, Changsha, Hunan Province 410007, China.
  • Chen CT; College of Acupuncture and Tuina and Rehabilitation, Hunan University of Traditional Chinese Medicine, Changsha, Hunan Province 410007, China. Electronic address: 314458702@qq.com.
  • Tian HM; College of Acupuncture and Tuina and Rehabilitation, Hunan University of Traditional Chinese Medicine, Changsha, Hunan Province 410007, China. Electronic address: 451358104@qq.com.
Brain Res Bull ; 215: 111031, 2024 Sep.
Article em En | MEDLINE | ID: mdl-39002935
ABSTRACT
We have previously reported that the expression of miR-34c-5p was up-regulated during acupuncture treatment in the setting of a cerebral ischemia/reperfusion injury (CIRI), indicating that miR-34c-5p plays an important role in healing from a CIRI-induced brain injury. This study sought to evaluate the effects of acupuncture on miR-34c-5p expression and autophagy in the forward and reverse directions using a rat focal cerebral ischemia/reperfusion model. After 120 minutes of middle cerebral artery occlusion and reperfusion, rats were treated with acupuncture at the "Dazhui" (DU20), "Baihui" (DU26) and "Renzhong" (DU14) points. Neurologic function deficit score, cerebral infarct area ratio, neuronal apoptosis and miR-34c-5p expression were evaluated 72 hr after treatment. The autophagy agonist RAPA and the antagonist 3MA were used to evaluate the neuro protective effects of autophagy-mediated acupuncture. We found that acupuncture treatment improved autophagy in the brain tissue of CIRI rats. Acupuncture reversed the negative effects of 3MA on CIRI, and acupuncture combined with RAPA further enhanced autophagy. We also found that acupuncture could increase miR-34c-5p expression in hippocampal neurons after ischemia/reperfusion. Acupuncture and a miR-34c agomir were able to enhance autophagy, improve neurologic deficits, and reduce the cerebral infarct area ratio and apoptosis rate by promoting the expression of miR-34c-5p. Silencing miR-34c resulted in a significantly reduced activating effect of acupuncture on autophagy and increased apoptosis, neurologic deficit symptoms, and cerebral infarct area ratio. This confirms that acupuncture can upregulate miR-34c-5p expression, which is beneficial in the treatment of CIRI.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Autofagia / Traumatismo por Reperfusão / Terapia por Acupuntura / Isquemia Encefálica / Ratos Sprague-Dawley / MicroRNAs Limite: Animals Idioma: En Revista: Brain Res Bull Ano de publicação: 2024 Tipo de documento: Article País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Autofagia / Traumatismo por Reperfusão / Terapia por Acupuntura / Isquemia Encefálica / Ratos Sprague-Dawley / MicroRNAs Limite: Animals Idioma: En Revista: Brain Res Bull Ano de publicação: 2024 Tipo de documento: Article País de publicação: Estados Unidos