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Use of Electron Microscopy for the Detection of Contaminant Endophytic Bacteria In Vitro.
de Araújo Silva-Cardoso, Inaê Mariê; Medeiros, Mariana Oliveira; Gomes, Ana Cristina Meneses Mendes; de Souza, André Luis Xavier; Scherwinski-Pereira, Jonny Everson.
Afiliação
  • de Araújo Silva-Cardoso IM; CNPq/Embrapa Postoctoral Fellowship Program, Embrapa Genetic Resources and Biotechnology, Brasília, DF, Brazil.
  • Medeiros MO; Department of Botany, University of Brasília, Campus Universitário Darcy Ribeiro, Brasília, DF, Brazil.
  • Gomes ACMM; Embrapa Genetic Resources and Biotechnology, PqEB, Brasília, DF, Brazil.
  • de Souza ALX; Embrapa Genetic Resources and Biotechnology, PqEB, Brasília, DF, Brazil.
  • Scherwinski-Pereira JE; Embrapa Genetic Resources and Biotechnology, PqEB, Brasília, DF, Brazil. jonny.pereira@embrapa.br.
Methods Mol Biol ; 2827: 71-84, 2024.
Article em En | MEDLINE | ID: mdl-38985263
ABSTRACT
The success of in vitro cultivation, particularly for micropropagation purposes, depends on the efficient control of contaminants. In this context, the sterilization of plant material constitutes a fundamental step in initiating cultures. Microbial contaminants can be found either on the surface (epiphyte) or inside plant explants (endophyte). However, the latter is generally challenging to detect and may not always be eradicated through surface sterilization alone. Endophyte contaminants, such as bacteria, can persist within plant material over several cultivation cycles, potentially interfering with or inhibiting in vitro establishment, growth, or recovery of cryopreserved materials. Therefore, microscopy techniques, such as electron microscopy, can yield valuable insights into bacterial endophytes' localization, tissue colonization patterns, and functions in in vitro plant culture. This information is essential for adopting effective strategies for eliminating, preventing, or harmonious coexistence with contaminants.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bactérias / Endófitos Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bactérias / Endófitos Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos