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Cardiac Development Long Non-Coding RNA (CARDEL) Is Activated during Human Heart Development and Contributes to Cardiac Specification and Homeostasis.
Pereira, Isabela T; Gomes-Júnior, Rubens; Hansel-Frose, Aruana; França, Rhaíza S V; Liu, Man; Soliman, Hossam A N; Chan, Sunny S K; Dudley, Samuel C; Kyba, Michael; Dallagiovanna, Bruno.
Afiliação
  • Pereira IT; Basic Stem Cell Biology Laboratory, Instituto Carlos Chagas-FIOCRUZ-PR, Curitiba 81350-010, PR, Brazil.
  • Gomes-Júnior R; Basic Stem Cell Biology Laboratory, Instituto Carlos Chagas-FIOCRUZ-PR, Curitiba 81350-010, PR, Brazil.
  • Hansel-Frose A; Basic Stem Cell Biology Laboratory, Instituto Carlos Chagas-FIOCRUZ-PR, Curitiba 81350-010, PR, Brazil.
  • França RSV; Basic Stem Cell Biology Laboratory, Instituto Carlos Chagas-FIOCRUZ-PR, Curitiba 81350-010, PR, Brazil.
  • Liu M; Department of Medicine, Division of Cardiology, University of Minnesota, Minneapolis, MN 55455, USA.
  • Soliman HAN; Lillehei Heart Institute, University of Minnesota, Minneapolis, MN 55455, USA.
  • Chan SSK; Lillehei Heart Institute, University of Minnesota, Minneapolis, MN 55455, USA.
  • Dudley SC; Department of Pediatrics, University of Minnesota, Minneapolis, MN 55455, USA.
  • Kyba M; Lillehei Heart Institute, University of Minnesota, Minneapolis, MN 55455, USA.
  • Dallagiovanna B; Department of Pediatrics, University of Minnesota, Minneapolis, MN 55455, USA.
Cells ; 13(12)2024 Jun 18.
Article em En | MEDLINE | ID: mdl-38920678
ABSTRACT
Successful heart development depends on the careful orchestration of a network of transcription factors and signaling pathways. In recent years, in vitro cardiac differentiation using human pluripotent stem cells (hPSCs) has been used to uncover the intricate gene-network regulation involved in the proper formation and function of the human heart. Here, we searched for uncharacterized cardiac-development genes by combining a temporal evaluation of human cardiac specification in vitro with an analysis of gene expression in fetal and adult heart tissue. We discovered that CARDEL (CARdiac DEvelopment Long non-coding RNA; LINC00890; SERTM2) expression coincides with the commitment to the cardiac lineage. CARDEL knockout hPSCs differentiated poorly into cardiac cells, and hPSC-derived cardiomyocytes showed faster beating rates after controlled overexpression of CARDEL during differentiation. Altogether, we provide physiological and molecular evidence that CARDEL expression contributes to sculpting the cardiac program during cell-fate commitment.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Diferenciação Celular / Miócitos Cardíacos / RNA Longo não Codificante / Coração / Homeostase Limite: Humans Idioma: En Revista: Cells Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Diferenciação Celular / Miócitos Cardíacos / RNA Longo não Codificante / Coração / Homeostase Limite: Humans Idioma: En Revista: Cells Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça