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The coculture of in vitro produced porcine embryos and oviductal epithelial cells improves blastocyst formation and modify embryo quality.
Lorenzo, Maria Soledad; Teplitz, Gabriela Maia; Luchetti, Carolina Griselda; Cruzans, Paula Romina; Bertonazzi, Analia; Lombardo, Daniel Marcelo.
Afiliação
  • Lorenzo MS; CONICET. Comisión Nacional de Investigaciones Científicas y Técnicas., Buenos Aires, Argentina; Universidad de Buenos Aires, Facultad de Ciencias Veterinarias, Instituto de Investigación y Tecnología en Reproducción Animal, Chorroarin 280, CABA, Argentina.
  • Teplitz GM; Universidad de Buenos Aires, Facultad de Ciencias Veterinarias, Instituto de Investigación y Tecnología en Reproducción Animal, Chorroarin 280, CABA, Argentina.
  • Luchetti CG; CONICET. Comisión Nacional de Investigaciones Científicas y Técnicas., Buenos Aires, Argentina; Universidad de Buenos Aires, Facultad de Ciencias Veterinarias, Instituto de Investigación y Tecnología en Reproducción Animal, Chorroarin 280, CABA, Argentina.
  • Cruzans PR; Universidad de Buenos Aires, Facultad de Ciencias Veterinarias, Instituto de Investigación y Tecnología en Reproducción Animal, Chorroarin 280, CABA, Argentina.
  • Bertonazzi A; Universidad de Buenos Aires, Facultad de Ciencias Veterinarias, Instituto de Investigación y Tecnología en Reproducción Animal, Chorroarin 280, CABA, Argentina.
  • Lombardo DM; CONICET. Comisión Nacional de Investigaciones Científicas y Técnicas., Buenos Aires, Argentina; Universidad de Buenos Aires, Facultad de Ciencias Veterinarias, Instituto de Investigación y Tecnología en Reproducción Animal, Chorroarin 280, CABA, Argentina. Electronic address: dlombard@fvet.uba.ar.
Theriogenology ; 226: 141-150, 2024 Sep 15.
Article em En | MEDLINE | ID: mdl-38885555
ABSTRACT
The efficiency of in vitro embryo production in mammals is influenced by variables associated with culture conditions during maturation, fertilization, and embryonic development. The embryos obtained often exhibit low quality due to suboptimal in vitro culture conditions compared to the in vivo environment. Co-culturing gametes and embryos with somatic cells has been developed to enhance in vitro culture conditions. This study aimed to assess the impact of coculturing in vitro-produced porcine embryos with porcine oviductal epithelial cells (POEC) on embryo development and quality. Firstly, a pure culture of POEC suitable for coculture systems was established. The epithelial origin of the cells was confirmed by the expression of E-cadherin and cytokeratin. The expression pattern of hormone receptors aligned with the diestrous oviduct, and POEC also secreted oviductal glycoprotein type 1 (OVGP-1). Secondly, POEC from passage 1 (POEC-1) were used to coculture with in vitro-produced porcine embryos. A successful coculture system was established without the addition of fetal bovine serum as a supplement. Coculturing POEC-1 in monolayers with in vitro-produced porcine embryos during the initial two days of culture enhanced the percentage of blastocysts and their hatching. Although the coculture did not alter the number of cells in the blastocysts or apoptosis assessed by TUNEL, it significantly reduced reactive oxygen species (ROS) levels in cleaved porcine embryos. This study represents the first report evaluating the quality of porcine embryos produced by IVF in coculture systems and assessing ROS levels in cleaved porcine embryos obtained by IVF.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Blastocisto / Fertilização in vitro / Técnicas de Cocultura / Técnicas de Cultura Embrionária / Células Epiteliais Limite: Animals Idioma: En Revista: Theriogenology Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Argentina País de publicação: Estados Unidos
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Blastocisto / Fertilização in vitro / Técnicas de Cocultura / Técnicas de Cultura Embrionária / Células Epiteliais Limite: Animals Idioma: En Revista: Theriogenology Ano de publicação: 2024 Tipo de documento: Article País de afiliação: Argentina País de publicação: Estados Unidos